Cardellini Jacopo, Balestri Arianna, Comparini Luca, Lonetti Barbara, Brucale Marco, Valle Francesco, Berti Debora, Montis Costanza
Department of Chemistry, University of Florence, and CSGI, Florence, Italy.
Laboratoire des IMRCP, Université de Toulouse, CNRS, Toulouse 31062, France.
J Colloid Interface Sci. 2024 Jan 15;654(Pt B):848-858. doi: 10.1016/j.jcis.2023.10.082. Epub 2023 Oct 18.
Hybrid lipid membranes incorporating amphiphilic copolymers have gained significant attention due to their potential applications in various fields, including drug delivery and sensing. By combining the properties of copolymers and lipid membranes, such as enhanced chemical tunability and stability, environmental responsiveness, and multidomain nature, novel membrane architectures have been proposed. In this study, we investigated the potentialities of hybrid membranes made of two distinct components: the rigid fully saturated phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and the soft copolymer poly(butadiene-b-ethyleneoxide) (PBD-b-PEO). The objective was to explore the interaction of citrate-coated gold nanoparticles (AuNPs) and the hybrid membrane, aiming at constructing AuNPs-hybrid vesicles suprastructures with controlled and adjustable plasmonic properties. A series of experimental techniques were employed to investigate hybrid free-standing and supported membranes. The results revealed that the incorporation of the copolymer into the lipid membrane promotes AuNPs clustering, demonstrating a distinctive aggregative phenomenon of citrate-coated AuNPs on multidomain membranes. Importantly, we show that the size and morphology of AuNPs clusters can be precisely controlled in non-homogeneous membranes, enabling the formation of hybrid suprastructures with controlled patch properties. These results highlight the potential of lipid-copolymer hybrid membranes for designing functional materials with tailored plasmonic properties, with potential applications in nanomedicine and sensing.
包含两亲性共聚物的混合脂质膜因其在包括药物递送和传感在内的各个领域的潜在应用而受到了广泛关注。通过结合共聚物和脂质膜的特性，如增强的化学可调性和稳定性、环境响应性以及多域性质，人们提出了新颖的膜结构。在本研究中，我们研究了由两种不同成分制成的混合膜的潜力：刚性的全饱和磷脂1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）和柔软的共聚物聚（丁二烯-b-环氧乙烷）（PBD-b-PEO）。目的是探索柠檬酸盐包覆的金纳米颗粒（AuNPs）与混合膜之间的相互作用，旨在构建具有可控和可调等离子体性质的AuNPs-混合囊泡超结构。我们采用了一系列实验技术来研究混合的独立膜和支撑膜。结果表明，将共聚物掺入脂质膜中会促进AuNPs聚集，这表明柠檬酸盐包覆的AuNPs在多域膜上存在独特的聚集现象。重要的是，我们表明在非均匀膜中可以精确控制AuNPs簇的大小和形态，从而能够形成具有可控斑块性质的混合超结构。这些结果突出了脂质-共聚物混合膜在设计具有定制等离子体性质的功能材料方面的潜力，在纳米医学和传感领域具有潜在应用。
