Catania Rosa, Heath George R, Rappolt Michael, Muench Stephen P, Beales Paul A, Jeuken Lars J C
School of Chemistry, University of Leeds Leeds LS2 9JT UK
Astbury Centre for Structural Molecular Biology, University of Leeds Leeds LS2 9JT UK.
RSC Appl Interfaces. 2025 Feb 11;2(3):665-672. doi: 10.1039/d4lf00362d. eCollection 2025 May 14.
Hybrid membranes, consisting of phospholipids and amphiphilic block polymers, offer enhanced stability compared to liposomes and greater biocompatibility than polymersomes. These qualities make them a versatile platform for a wide range of applications across various fields. In this study, we have investigated the ability of solid-supported polymer-lipid hybrid membranes (SSHM) to act as a platform for bioelectrochemistry of membrane proteins. The redox enzyme, cytochrome (cyt ), a terminal oxidase in , was reconstituted into hybrid vesicles (HVs), which were subsequently tested for their ability to form SSHMs on different self-assembled monolayers (SAMs) on gold electrodes. SSHM formation was monitored with electrochemical impedance spectroscopy (EIS), quartz crystal microbalance with dissipation (QCM-D), and atomic force microscopy (AFM). SSHMs were successfully formed on gold electrodes with mixed SAMs of 6-mercapto-1-hexanol and 1-hexanethiol at a 1 : 1 ratio. The activity of cyt was confirmed using cyclic voltammetry (CV), with electron transfer to cyt mediated by a lipophilic substrate-analogue decylubiquinone (DQ). SSHMs formed with HVs-cyt samples, stored for more than one year before use, remain bioelectrocatalytically active, confirming our previously established longevity and stability of HV systems.
由磷脂和两亲性嵌段聚合物组成的混合膜，与脂质体相比具有更高的稳定性，与聚合物囊泡相比具有更好的生物相容性。这些特性使其成为跨领域广泛应用的通用平台。在本研究中，我们研究了固体支持的聚合物 - 脂质混合膜（SSHM）作为膜蛋白生物电化学平台的能力。氧化还原酶细胞色素c（cyt c），作为呼吸链中的末端氧化酶，被重组到混合囊泡（HV）中，随后测试其在金电极上不同自组装单分子层（SAM）上形成SSHM的能力。用电化学阻抗谱（EIS）、耗散型石英晶体微天平（QCM - D）和原子力显微镜（AFM）监测SSHM的形成。在金电极上成功形成了由6 - 巯基 - 1 - 己醇和1 - 己硫醇以1∶1比例组成的混合SAM的SSHM。使用循环伏安法（CV）确认了cyt c的活性，电子通过亲脂性底物类似物癸基泛醌（DQ）介导转移到cyt c。由HVs - cyt c样品形成的SSHM，在使用前储存了一年多，仍保持生物电催化活性，证实了我们之前建立的HV系统的寿命和稳定性。
