Nergiz Yusuf, Deveci Engin, Sak Erdal, Evsen Sıddık, Tunik Selçuk, Nergiz Şebnem, Aşır Fırat, Şeker Uğur
Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakır 21280, Turkey.
Department of Obstetrics and Gynecology, Faculty of Medicine, Dicle University, Diyarbakır 21280, Turkey.
Asian Biomed (Res Rev News). 2023 Oct 26;17(5):238-243. doi: 10.2478/abm-2023-0065. eCollection 2023 Oct.
Hemolysis, elevated liver enzymes, low platelet count (HELLP) syndrome is generally considered to be a variant or complication of preeclampsia. It is a life-threatening obstetric complication.
To evaluate the immunohistochemistry and ultrastructural of syncytiotrophoblastand Hoffbauer cells in placental villi of patients with HELLP syndrome.
Two groups of patients with a total of 50 full-term human placentas (n = 25 in each group) were assigned as the control (normotensive) and HELLP syndrome. Placental tissue samples were fixed in 10% neutral formalin and paraffin-embedding protocol was performed. We prepared 5 μm sections for histological and immunohistochemical staining. Sections were immunostained with Hoffbauer cell marker CD68. For transmission electron microscopy (TEM), placental tissue samples were fixed in 2.5% buffered glutaraldehyde and then, in 1% osmium tetroxide for routine ultrastructural examinations.
When the HELLP group fetal placental sections were examined, intracytoplasmic edema in syncytiotrophoblast, degenerative vacuoles, and degenerative findings on cell surface membranes were observed. Moreover, villous edema was remarkable. The number of CD68-positive Hoffbauer cells per villus control group sections was 0.23 ± 0.02 and the number of CD68-positive cells per villus in HELLP group placenta sections was 0.83 ± 0.12. The increase in the number of Hoffbauer cells per villus in the HELLP group was significant ( < 0.001). Compared with the control group, there was a significant increase in the number of Hoffbauer cells and syncytiotrophoblasts in the HELLP group, and degenerative changes were also observed in the ultrastructure of these cells.
Pathology of the HELLP syndrome is in relation to CD68-positive placental macrophages.
溶血、肝酶升高、血小板减少（HELLP）综合征通常被认为是子痫前期的一种变体或并发症。它是一种危及生命的产科并发症。
评估HELLP综合征患者胎盘绒毛中合体滋养层细胞和霍夫鲍尔细胞的免疫组化及超微结构。
将两组共50例足月人胎盘（每组n = 25）分别作为对照组（血压正常）和HELLP综合征组。胎盘组织样本用10%中性福尔马林固定，并进行石蜡包埋。我们制备了5μm切片用于组织学和免疫组化染色。切片用霍夫鲍尔细胞标志物CD68进行免疫染色。对于透射电子显微镜（TEM），胎盘组织样本用2.5%缓冲戊二醛固定，然后用1%四氧化锇固定用于常规超微结构检查。
检查HELLP组胎儿胎盘切片时，观察到合体滋养层细胞胞浆水肿、退行性空泡以及细胞表面膜的退行性改变。此外，绒毛水肿明显。对照组切片每绒毛CD68阳性霍夫鲍尔细胞数为0.23±0.02，HELLP组胎盘切片每绒毛CD68阳性细胞数为0.83±0.12。HELLP组每绒毛霍夫鲍尔细胞数的增加具有显著性（<0.001）。与对照组相比，HELLP组霍夫鲍尔细胞和合体滋养层细胞数量显著增加，并且这些细胞的超微结构也观察到退行性改变。
HELLP综合征的病理学与CD68阳性胎盘巨噬细胞有关。
