McGowan J W, Borwein B, Medeiros J A, Beveridge T, Brown J D, Spiller E, Feder R, Topalian J, Gudat W
J Cell Biol. 1979 Mar;80(3):732-5. doi: 10.1083/jcb.80.3.732.
High resolution x-ray lithographic studies of cells from chick embryo hearts dried by the CO2 critical point method have been made with soft x-ray radiation of different wavelengths. A marked difference in the relief replica in polymethyl methacrylate (PMMA) resulting from the differential absorption by the dried cells of carbon K alpha radiation at 4.48 nm and broad band synchrotron radiation (SR) with lambda is greater than 1.5 nm demonstrates the potential usefulness of the technique in making high resolution (approximately or equal to 10 nm) chemical identification of the constitutents which make up the various parts of the cell.
利用不同波长的软X射线辐射,对采用二氧化碳临界点干燥法干燥的鸡胚心脏细胞进行了高分辨率X射线光刻研究。由4.48纳米的碳Kα辐射及波长大于1.5纳米的宽带同步辐射(SR)在干燥细胞中的差异吸收所导致的聚甲基丙烯酸甲酯(PMMA)浮雕复制品中的显著差异,证明了该技术在对构成细胞各个部分的成分进行高分辨率(约等于10纳米)化学识别方面的潜在用途。