Department of Allied Health Resources, State University of New York, Stony Brook, 11794, Stony Brook, NY.
Biol Trace Elem Res. 1987 Apr;12(1):167-83. doi: 10.1007/BF02796678.
Soft X-ray contact microscopy with synchrotron radiation offers the biologist, and especially the microscopist, a way to morphologically study specimens that could not be imaged by conventional TEM, STEM, or SEM methods (i.e., hydrated samples, samples easily damaged by an electron beam, electron-dense samples, thick specimens, unstained, low-contrast specimens) at spatial resolutions approaching those of the TEM, with the additional possibility to obtain compositional (elemental) information about the sample as well. Although flash X-ray sources offer faster exposure times, synchrotron radiation provides a highly collimated, intense radiation that can be tuned to select specific discrete ranges of X-ray wavelengths or specific individual wavelengths that optimize imaging or microanalysis of a specific sample. This paper presents an overview of the applications of X-ray contact microscopy to biological research and some current research results using monochromatic synchrotron radiation to image biological samples.
同步辐射软 X 射线接触显微镜为生物学家，特别是显微镜学家提供了一种方法，可以对传统的 TEM、STEM 或 SEM 方法无法成像的标本进行形态学研究（即，水合样品、容易被电子束损坏的样品、电子密度高的样品、厚标本、未染色、低对比度标本），其空间分辨率接近 TEM，并且还可以获得有关样品的组成（元素）信息。虽然闪光 X 射线源提供了更快的曝光时间，但同步辐射提供了高度准直、强度高的辐射，可以调整为选择特定离散范围的 X 射线波长或特定的单个波长，以优化特定样品的成像或微分析。本文介绍了 X 射线接触显微镜在生物研究中的应用概述，并介绍了一些当前使用单色同步辐射对生物样品进行成像的研究结果。
