Goldman R D, Chojnacki B, Yerna M J
J Cell Biol. 1979 Mar;80(3):759-66. doi: 10.1083/jcb.80.3.759.
After standard glutaraldehyde-osmium tetroxide fixation procedures, the majority of microfilament bundles in BHK-21 cells exhibit relatively uniform electron density along their long axes. The inclusion of tannic acid in the glutaraldehyde fixation solution results in obvious electron density shifts along the majority of microfilament bundles. Striated patterens are frequently observed which consist of regularly spaced electron dense (D) and electron lucid (L) bands. A striated pattern is also observed along many BHK-21 stress fibers after processing for indirect immunofluorescence utilizing BHK-21 myosin antiserum. A direct correlation of these periodicities seen by light and electron microscope techniques is impossible at the present time. However, comparative measurements indicate that the overall patterns seen in the immunofluorescence and electron microscope preparations are similar. The ultrastructural results provide an initial clue for the ultimate determination of the supramolecular organization of contracile proteins other than actin within the microfilament bundles of non-muscle cells.
经过标准的戊二醛-四氧化锇固定程序后，BHK-21细胞中的大多数微丝束沿其长轴呈现相对均匀的电子密度。在戊二醛固定液中加入单宁酸会导致大多数微丝束出现明显的电子密度变化。经常观察到条纹状图案，其由规则间隔的电子致密（D）带和电子透明（L）带组成。在用BHK-21肌球蛋白抗血清进行间接免疫荧光处理后，在许多BHK-21应力纤维上也观察到条纹状图案。目前，通过光学显微镜和电子显微镜技术观察到的这些周期性之间无法建立直接关联。然而，对比测量表明，免疫荧光和电子显微镜制备中观察到的整体图案相似。超微结构结果为最终确定非肌肉细胞微丝束中除肌动蛋白外的收缩蛋白的超分子组织提供了初步线索。
