韩国马品种亲子鉴定的单核苷酸多态性

Single nucleotide polymorphisms for parentage testing of horse breeds in Korea.

作者信息

Lee Sun-Young, Kim Su-Min, Oyungerel Baatartsogt, Cho Gil-Jae

机构信息

Racing Laboratory, Korea Racing Authority, Gwacheon 13822, Korea.

Department of Horse Industry, Sung Woon University, Yeongcheon 38801, Korea.

出版信息

Anim Biosci. 2024 Apr;37(4):600-608. doi: 10.5713/ab.23.0123. Epub 2023 Oct 27.

DOI:10.5713/ab.23.0123

PMID:37905321

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10915227/

Abstract

OBJECTIVE

In this study, we aimed to evaluate the usability single nucleotide polymorphisms (SNPs) for parentage testing of horse breeds in Korea.

METHODS

The genotypes of 93 horse samples (38 Thoroughbred horses, 17 Jeju horses, 20 Quarter horses, and 18 American miniature horses) were determined using 15 microsatellite (Ms) markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3, and VHL20) and 101 SNP markers.

RESULTS

Paternity tests were performed using 15 Ms markers and 101 SNP markers in Thoroughbred horses and Quarter horses. AHT5, ASB2, ASB17, ASB23, CA425, HMS7, HTG10, and LEX3 did not follow Mendelian inheritance in Thoroughbred horses, whereas in Quarter horses, only AHT4, ASB2, and HMS2 showed Mendelian inheritance, consequently, paternity was not established. Meanwhile, 31 markers, including MNEc_2_2_ 2_98568918_BIEC2_502451, in Thoroughbred horses, and 30 markers, including MNEc_ 2_30_7430735_BIEC2_816793, in Quarter horses did not conform with Mendelian inheritance and therefore, could not be used for establishing parentage.

CONCLUSION

The possibility of replacing Ms markers with SNP markers for paternity testing in horses was confirmed. However, further research using more samples is necessary.

摘要

目的

在本研究中，我们旨在评估用于韩国马品种亲子鉴定的单核苷酸多态性（SNP）的可用性。

方法

使用15个微卫星（Ms）标记（AHT4、AHT5、ASB2、ASB17、ASB23、CA425、HMS1、HMS2、HMS3、HMS6、HMS7、HTG4、HTG10、LEX3和VHL20）和101个SNP标记确定了93匹马样本（38匹纯种马、17匹济州马、20匹夸特马和18匹美国迷你马）的基因型。

结果

在纯种马和夸特马中使用15个Ms标记和101个SNP标记进行亲子鉴定。在纯种马中，AHT5、ASB2、ASB17、ASB23、CA425、HMS7、HTG10和LEX3不遵循孟德尔遗传，而在夸特马中，只有AHT4、ASB2和HMS2显示孟德尔遗传，因此，亲子关系未确立。同时，纯种马中的31个标记，包括MNEc_2_2_2_98568918_BIEC2_502451，以及夸特马中的30个标记，包括MNEc_2_30_7430735_BIEC2_816793，不符合孟德尔遗传，因此不能用于确立亲子关系。