Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, 3619995161, Iran.
Department of Horticulture Science and Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, 3619995161, Iran.
Sci Rep. 2023 Oct 31;13(1):18672. doi: 10.1038/s41598-023-45823-8.
The pretreatment of seeds with cold plasma (CP) (0 and 100 w for 240 s), and salicylic acid priming (SA) (0 and 2 mM normal and nano form), and foliar spraying of SA at the six-leaf stage (0 and 2 mM normal and nano form) of Salvia leriifolia plants in field condition was studied. Compared to the control plants of S. leriifolia, the results showed that CP + both forms of SA priming + nano-SA spraying increased plant height, leaf length, plant dry weight, total phenol, and the activities of phenylalanine ammonia-lyase (PAL) and tyrosine ammonia-lyase (TAL) enzymes. The chlorophyll a and b contents in all treated plants remained either unchanged or decreased when compared to the control. The highest PAL activity was obtained in CP-free + hydro-priming + nano-SA foliar spraying. The highest content of caffeic acid was achieved in CP + SA priming + SA foliar spraying in the leaf. The maximum contents of rosmarinic and salvianolic acid were obtained in the control plants. In conclusion, CP and nano-SA can increase PAL and TAL activity and total phenol accumulation in S. leriifolia plants, but not rosmarinic and salvianolic acid contents. Other phenolic compound enzymes and their production require further study.
在田间条件下，研究了用冷等离子体（CP）（0 和 100 W，240 s）预处理种子，水杨酸引发（SA）（0 和 2 mM 正常和纳米形式），以及在六叶期叶面喷施 SA（0 和 2 mM 正常和纳米形式）对薰衣草植物的影响。与薰衣草植物的对照植株相比，结果表明，CP+两种形式的 SA 引发+纳米 SA 喷雾增加了株高、叶长、植株干重、总酚以及苯丙氨酸氨裂解酶（PAL）和酪氨酸氨裂解酶（TAL）的活性。与对照相比，所有处理植株的叶绿素 a 和 b 含量保持不变或降低。无 CP+水引发+纳米 SA 叶面喷雾的 PAL 活性最高。在叶片中，CP+SA 引发+SA 叶面喷雾获得了最高的咖啡酸含量。迷迭香酸和丹酚酸 B 的最大含量在对照植株中获得。总之，CP 和纳米 SA 可以增加 S. leriifolia 植物中 PAL 和 TAL 的活性以及总酚的积累，但不能增加迷迭香酸和丹酚酸 B 的含量。其他酚类化合物酶及其产物需要进一步研究。
