Sheteiwy Mohamed S, An Jianyu, Yin Mengqi, Jia Xiaowen, Guan Yajing, He Fei, Hu Jin
Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Department of Agronomy, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
Protoplasma. 2019 Jan;256(1):79-99. doi: 10.1007/s00709-018-1279-0. Epub 2018 Jul 8.
The present study was designed to highlight the effects of cold plasma (10 kV) treatment and priming with 2 mM salicylic acid (SA) and their combination (10 kV of plasma + 2 mM SA) on the physiological parameters and metabolism of two cultivars of Oryza sativa, i.e., Zhu Liang You 06 (ZY) and Qian You No. 1 (QY), under salinity stress (150 mM NaCl) and normal growth condition (0 mM NaCl). Seed germination and seedling growth were enhanced by SA priming and cold plasma treatment either alone or in combination under salinity stress. Photosynthetic pigments, photosynthetic gas exchange, and chlorophyll fluorescence were improved by cold plasma treatment and SA priming under salinity stress as compared to the untreated seeds. The activities of antioxidant enzymes were significantly improved by the combination of SA priming and cold plasma treatment in both cultivars under salinity stress. There were rapid changes in the cellular content of sodium (Na) and calcium (Ca), where the plants grown under saline conditions accumulate more Na and less Ca contents resulting in ionic imbalances. Interestingly, cold plasma and SA treatments diminished this action by reducing Na accumulation and increasing K and Ca contents in the plant cell under salinity stress. The activities of enzymes involved in secondary metabolism assimilation were up-regulated with cold plasma and SA priming either alone or combination under salinity stress. An increase in reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content was also observed under salinity stress condition. On contrast, seed treated with SA and plasma alone or combined resulted in a significant decrease in ROS and MDA contents under salinity stress. Our results indicated that SA priming and cold plasma treatment either alone or combined improved plant uptake of nutrients in both cultivars under stress conditions. The ultrastructural changes were observed to be more prominent in ZY than QY cultivar. Plants without SA priming or cold plasma treatments have a big vacuole due to the movement of ions into the vacuole directly from the apoplast into the vacuole through membrane vesiculation leading to membrane destabilization. However, SA priming and cold plasma treatment alone or combined helped the plants to recover their cell turgidity under salinity stress.
本研究旨在突出冷等离子体（10 kV）处理以及用2 mM水杨酸（SA）引发及其组合（10 kV等离子体 + 2 mM SA）对两个水稻品种，即株两优06（ZY）和钱优1号（QY），在盐胁迫（150 mM NaCl）和正常生长条件（0 mM NaCl）下生理参数和代谢的影响。在盐胁迫下，单独或组合使用SA引发和冷等离子体处理均可提高种子萌发和幼苗生长。与未处理的种子相比，在盐胁迫下，冷等离子体处理和SA引发可改善光合色素、光合气体交换和叶绿素荧光。在盐胁迫下，两个品种中SA引发和冷等离子体处理的组合均显著提高了抗氧化酶的活性。钠（Na）和钙（Ca）的细胞含量发生了快速变化，在盐条件下生长的植物积累更多的Na且Ca含量较少，导致离子失衡。有趣的是，冷等离子体和SA处理通过在盐胁迫下减少植物细胞中Na的积累并增加K和Ca含量，减轻了这种作用。在盐胁迫下，单独或组合使用冷等离子体和SA引发会上调参与次生代谢同化的酶的活性。在盐胁迫条件下，还观察到活性氧（ROS）积累和丙二醛（MDA）含量增加。相比之下，单独或组合用SA和等离子体处理的种子在盐胁迫下导致ROS和MDA含量显著降低。我们的结果表明，单独或组合使用SA引发和冷等离子体处理可改善胁迫条件下两个品种植物对养分的吸收。观察到超微结构变化在ZY品种中比QY品种更明显。未经SA引发或冷等离子体处理的植物由于离子通过膜泡化直接从质外体进入液泡导致膜不稳定，从而具有大液泡。然而，单独或组合使用SA引发和冷等离子体处理有助于植物在盐胁迫下恢复细胞膨压。
