Barra A, Aucouturier P, Preud'homme J L
Diagn Immunol. 1986;4(5):228-32.
The subclass distribution of human anti-thyroglobulin IgG antibodies (TgAb) was studied in 24 sera using an indirect enzyme-linked immunosorbent assay (ELISA) with monoclonal anti-subclass antibodies. An IgG4/IgG1 predominance was found. TgAb were purified from seven sera by affinity chromatography. The antigen-specific indirect ELISA was repeated in the purified TgAb, in which IgG subclasses were measured by a competitive assay with monoclonal antibodies. The latter method showed an IgG2 predominance. Comparison of the results obtained by the three approaches showed discrepancies that probably reflect bias introduced by both the indirect ELISA and the affinity chromatography.
使用单克隆抗亚类抗体的间接酶联免疫吸附测定（ELISA），对24份血清中的人抗甲状腺球蛋白IgG抗体（TgAb）亚类分布进行了研究。发现以IgG4/IgG1为主。通过亲和层析从7份血清中纯化TgAb。在纯化的TgAb中重复进行抗原特异性间接ELISA，其中通过与单克隆抗体的竞争测定法测量IgG亚类。后一种方法显示以IgG2为主。对三种方法获得的结果进行比较，发现存在差异，这可能反映了间接ELISA和亲和层析引入的偏差。
