Warr G W, Deluca D, Griffin B R
J Immunol. 1979 Aug;123(2):910-7.
Three rabbit antisera raised to trout IgM showed positive immunofluorescent reactions with lymphocytes of trout spleen and thymus. These antisera showed no detectable cross-reactivity with keyhole limpet hemocyanin, as determined by direct radioimmune precipitation and adsorption, and did not appear to react significantly with carbohydrate moieties on trout IgM, as determined by the failure of proteolytic digests of trout IgM to inhibit in radioimmunoassay against intact trout IgM. Membrane immunoglobulin determinants were detectable on the membrane of both thymocytes and splenocytes with the lactoperoxidase-catalyzed iodination reaction. Radioimmunoassay of lysates of lymphocytes confirmed the association of immunoglobulin with trout thymocytes. By radioimmunoassay, lysates of thymic and splenic lymphocytes contained similar amounts of immunoglobulin, equivalent to approximately 8 X 10(4) molecules of IgM per cell (calculated as the monomer micro 2L2).
三种针对鳟鱼免疫球蛋白M（IgM）产生的兔抗血清，与鳟鱼脾脏和胸腺的淋巴细胞呈现出阳性免疫荧光反应。通过直接放射免疫沉淀和吸附测定，这些抗血清与钥孔戚血蓝蛋白未显示出可检测到的交叉反应；并且，由于鳟鱼IgM的蛋白水解消化物未能在针对完整鳟鱼IgM的放射免疫测定中起到抑制作用，表明这些抗血清似乎与鳟鱼IgM上的碳水化合物部分没有明显反应。通过乳过氧化物酶催化的碘化反应，在胸腺细胞和脾细胞的膜上均可检测到膜免疫球蛋白决定簇。淋巴细胞裂解物的放射免疫测定证实了免疫球蛋白与鳟鱼胸腺细胞的关联。通过放射免疫测定，胸腺和脾脏淋巴细胞的裂解物含有相似量的免疫球蛋白，相当于每个细胞约8×10⁴个IgM分子（按单体μ2L2计算）。
