Warr G W, DeLuca D, Anderson D P
Comp Biochem Physiol B. 1983;76(3):515-21. doi: 10.1016/0305-0491(83)90285-7.
Thymic lymphocytes of the rainbow trout, S. gairdneri were disrupted and a plasma membrane containing fraction isolated by differential and buoyant density centrifugation. Radioiodine introduced into the membrane by the lactoperoxidase catalyzed reaction and immunoglobulin (identified by radioimmunoassay with monoclonal antibody) both copurified in the plasma membrane fraction. Rabbit antibody raised to the plasma membrane fraction showed a strong reaction with trout lymphocytes in immunofluorescence, was mitogenic for trout lymphocytes, and recognized lymphocyte membrane heteroantigens of molecular weight greater than 70,000 in the thymus and 45,000-95,000 in the head kidney.
虹鳟（S. gairdneri）的胸腺淋巴细胞被破坏，通过差速离心和浮力密度离心分离出含质膜的部分。通过乳过氧化物酶催化反应引入膜中的放射性碘和免疫球蛋白（通过单克隆抗体放射免疫测定鉴定）都在质膜部分中共纯化。针对质膜部分产生的兔抗体在免疫荧光中与鳟鱼淋巴细胞有强烈反应，对鳟鱼淋巴细胞有促有丝分裂作用，并识别胸腺中分子量大于70,000以及头肾中分子量为45,000 - 95,000的淋巴细胞膜异种抗原。
