Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
The Dental College of Georgia, Augusta University, Augusta, GA, USA.
J Dent. 2024 Jan;140:104770. doi: 10.1016/j.jdent.2023.104770. Epub 2023 Nov 2.
To evaluate the bacteria debridement efficacy of two generations of sonic root canal irrigant activation systems: EndoActivator (Dentsply Sirona), the first generation, and SmartLite Pro EndoActivator, the second generation.
Instrumented, autoclaved, single-rooted human premolars were inoculated with Enterococcus faecalis (ATCC-29212) for 21 days. The bacteria biofilm-containing teeth were randomly divided into 5 groups (N=8): Group 1: Syringe-side-vented needle (S-N) delivery of saline for 1 min; Group 2: S-N delivery of 2% NaOCl for 1 min; Group 3: S-N delivery of 2% NaOCl for 5 min; Group 4: EndoActivator activation of 2% NaOCl for 1 min; Group 5: SmartLite Pro EndoActivator activation of 2% NaOCl for 1 min. The teeth were evaluated for bacterial reduction using CFU counts, and the percentages of dead bacteria within the dentinal tubules using confocal laser scanning microscopy.
Activation of NaOCl with EndoActivator or SmartLite Pro EndoActivator significantly reduced the overall intracanal bacterial load, compared with S-N irrigant delivery (P<0.05), with no significant difference between the two agitation devices (P>0.05). Nevertheless, S-N delivery of 2% NaOCl for 5 min produced better bacteria debridement than either sonic agitation system. Different degrees of bacteria kill were identified in the coronal-middle portions and apical portion of the canal space.
Delivery time of NaOCl affects the efficacy of bacteria disinfection. Activation for 1 min with the EndoActivator or SmartLite Pro EndoActivator demonstrated comparable canal wall biofilm and intracanal bacteria reduction efficacy when 2% NaOCl was used as irrigant for disinfecting E. faecalis in single-rooted teeth.
Although the sonic root canal irrigant activation devices investigated do not completely eliminate live bacteria biofilms from the canal space, they help reduce bacteria load during irrigant activation.
评估两代声波根管冲洗激活系统（一代 EndoActivator，登士柏西诺德；二代 SmartLite Pro EndoActivator）的细菌清创效果。
将经过器械预备、高压蒸汽灭菌的单根人前磨牙用粪肠球菌（ATCC-29212）接种 21 天。将含细菌生物膜的牙齿随机分为 5 组（N=8）：组 1：注射器侧孔针（S-N）输送生理盐水 1 分钟；组 2：S-N 输送 2%次氯酸钠 1 分钟；组 3：S-N 输送 2%次氯酸钠 5 分钟；组 4：EndoActivator 激活 2%次氯酸钠 1 分钟；组 5：SmartLite Pro EndoActivator 激活 2%次氯酸钠 1 分钟。使用 CFU 计数评估牙齿的细菌减少情况，使用共聚焦激光扫描显微镜评估牙本质小管内死亡细菌的百分比。
与 S-N 冲洗输送相比，使用 EndoActivator 或 SmartLite Pro EndoActivator 激活次氯酸钠可显著降低根管内整体细菌负荷（P<0.05），两种搅拌设备之间无显著差异（P>0.05）。然而，S-N 输送 2%次氯酸钠 5 分钟的清创效果优于任何一种声波搅拌系统。在根管的冠-中部分和根尖部分都观察到不同程度的细菌杀灭。
次氯酸钠的输送时间会影响细菌消毒的效果。在使用 2%次氯酸钠作为冲洗液对单根牙齿中的粪肠球菌进行消毒时，EndoActivator 或 SmartLite Pro EndoActivator 激活 1 分钟的效果与根管壁生物膜和根管内细菌减少的效果相当。
虽然所研究的声波根管冲洗激活装置并不能完全清除根管内的活细菌生物膜，但它们有助于在冲洗激活过程中减少细菌负荷。
