Department of Gastrointestinal Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China.
Int J Biol Macromol. 2024 Jan;254(Pt 1):127763. doi: 10.1016/j.ijbiomac.2023.127763. Epub 2023 Nov 2.
Gastric cancer is the fifth most frequently diagnosed malignant neoplasm and the third leading cause of cancer-related mortality. Nevertheless, the therapeutic efficacy of conventional surgical and chemotherapeutic interventions in clinical practice is often unsatisfactory. Curcumin (Cur) has shown promise as a therapeutic agent in prior studies. However, its progress in this context has been impeded by challenges including low solubility, instability in aqueous environments, and rapid metabolism. In this study, we develop methacrylate fish gelatin (FGMA) hydrogel microparticles (FGMPs@Cur) encapsulating Cur via microfluidic electrospray technology for postoperative comprehensive treatment of gastric cancer. Comprehensive characterizations and analyses were conducted to assess the cytotoxicity against gastric cancer cells and potential tissue reparative effects of FGMPs@Cur. In vitro experiments revealed that FGMPs@Cur exhibited a remarkable cytotoxic effect on nearly 80 % of gastric cancer cells while maintaining at least 95 % viability of normal cells in cell compatibility tests. In vivo results demonstrated that FGMPs@Cur significantly reduced tumor volume to 47 % of the control group, and notable tissue regeneration was observed at the surgical site. These properties indicated that such a hydrogel microparticle system is a promising candidate for postoperative gastric cancer treatment in practical application.
胃癌是第五种最常见的恶性肿瘤，也是癌症相关死亡的第三大主要原因。然而，在临床实践中，常规手术和化疗干预的治疗效果往往不尽人意。姜黄素（Cur）在先前的研究中已被证明是一种有前途的治疗剂。然而，其在这方面的进展受到了一些挑战的阻碍，包括低溶解度、在水介质中的不稳定性和快速代谢。在本研究中，我们通过微流控电喷技术开发了一种载有姜黄素的甲基丙烯酰化鱼明胶（FGMA）水凝胶微球（FGMPs@Cur），用于胃癌的术后综合治疗。我们对 FGMPs@Cur 进行了全面的表征和分析，以评估其对胃癌细胞的细胞毒性和潜在的组织修复作用。体外实验表明，FGMPs@Cur 对近 80%的胃癌细胞表现出显著的细胞毒性作用，而在细胞相容性试验中，正常细胞的存活率至少保持在 95%。体内结果表明，FGMPs@Cur 显著将肿瘤体积缩小到对照组的 47%，并且在手术部位观察到明显的组织再生。这些特性表明，这种水凝胶微球系统是一种很有前途的用于实际应用的术后胃癌治疗候选物。
