State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, Hubei, China.
College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, Hubei, China.
Anal Bioanal Chem. 2024 Jan;416(1):163-173. doi: 10.1007/s00216-023-05002-0. Epub 2023 Nov 6.
Alpha-fetoprotein (AFP) is inextricably linked to various diseases, including liver cancer. Thus, detecting the content of AFP in biology has great significance in diagnosis, treatment, and intervention. Motivated by the urgent need for affordable and convenient electronic sensors in the analysis and detection of aqueous biological samples, we combined the solution-gated graphene transistor (SGGT) with the catalytic reaction of enzyme nanoprobes (HRP-AuNPs-Ab2) to accurately sense AFP. The SGGT immunosensor demonstrated high specificity and stability, excellent selectivity, and excessive linearity over a range of 4 ng/mL to 500 ng/mL, with the lower detection limit down to 1.03 ng/mL. Finally, clinical samples were successfully detected by the SGGT immunosensor, and the results were consistent with chemiluminescence methods that are popular in hospitals for detecting AFP. Notably, the SGGT immunosensor is also recyclable, so it has excellent potential for use in high-throughput detection.
甲胎蛋白（AFP）与各种疾病，包括肝癌密切相关。因此，检测生物样品中甲胎蛋白的含量对于诊断、治疗和干预具有重要意义。受分析和检测水溶液生物样品中对价格合理且方便的电子传感器的迫切需求的驱动，我们将溶液门控石墨烯晶体管（SGGT）与酶纳米探针（HRP-AuNPs-Ab2）的催化反应相结合，以准确检测 AFP。SGGT 免疫传感器表现出高特异性和稳定性、优异的选择性和在 4ng/mL 至 500ng/mL 的范围内的超线性，检测下限低至 1.03ng/mL。最后，通过 SGGT 免疫传感器成功检测了临床样本，结果与医院中常用的检测 AFP 的化学发光法一致。值得注意的是，SGGT 免疫传感器还可回收利用，因此在高通量检测中具有优异的应用潜力。
