Sun Chenfang, Li Rui, Song Yaru, Jiang Xiaoqian, Zhang Congcong, Cheng Shanshan, Hu Wenping
Department of Chemistry, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, School of Science, Tianjin University, Tianjin 300072, China.
Institute for Advanced Interdisciplinary Research (iAIR), Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong, University of Jinan, Jinan 250011, China.
Anal Chem. 2021 Apr 20;93(15):6188-6194. doi: 10.1021/acs.analchem.1c00372. Epub 2021 Mar 29.
Organic field-effect transistors (OFETs) are considered as one of the cost-effective biosensor devices with rapid detection capabilities and multiparameter responses. However, the functionalization processes on normal organic devices might impact the device performance for its further sensitive and reliable sensing applications. Herein, we develop a novel organic material, 2,6-bis(4-formylphenyl)anthracene (BFPA) for use as the protective and functional layer of OFET-based biosensors, enabling ultrasensitive determination of alpha-fetoprotein (AFP) with femtomolar accuracy in human serum. By monitoring changes of the source-drain current () and threshold voltage () electrical signals, the device exhibits improved reliability in detecting AFP biomarkers and is able to differentiate between liver cancer patients and healthy individuals. Featuring label-free determination, shorter analysis time, and lower sample volume, this ultrasensitive and reliable OFET-based biosensor displays numerous advantages over traditional strategies such as enzyme-linked immunosorbent assay and electrochemiluminescence immunoassay, demonstrating that the proposed OFET-based biosensors have broader analytical and clinical applications for early liver cancer diagnosis.
有机场效应晶体管（OFET）被认为是具有快速检测能力和多参数响应的经济高效的生物传感器设备之一。然而，普通有机设备上的功能化过程可能会影响其进一步的灵敏和可靠传感应用的设备性能。在此，我们开发了一种新型有机材料2,6-双（4-甲酰基苯基）蒽（BFPA），用作基于OFET的生物传感器的保护和功能层，能够在人血清中以飞摩尔精度超灵敏地测定甲胎蛋白（AFP）。通过监测源漏电流（）和阈值电压（）电信号的变化，该设备在检测AFP生物标志物方面表现出更高的可靠性，并且能够区分肝癌患者和健康个体。这种基于OFET的超灵敏且可靠的生物传感器具有无需标记测定、分析时间短和样品体积小的特点，与酶联免疫吸附测定和电化学发光免疫测定等传统策略相比具有诸多优势，表明所提出的基于OFET的生物传感器在早期肝癌诊断方面具有更广泛的分析和临床应用。
