三叶准噶尔黄芪多糖对雏鸡生长及肝脏代谢的影响。
Effect of Pseudostellaria heterophylla polysaccharide on the growth and liver metabolism of chicks.
机构信息
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China.
出版信息
J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skad368.
In this study, the effects of Pseudostellaria heterophylla polysaccharide (PHP) on the growth, development, and liver metabolism of chicks were investigated by feeding chicks diets. Four hundred 7-d-old Gushi roosters were selected and randomly divided into four groups, labeled A, B, C, and D. Group A was fed the basal diet, and Groups B, C, and D were fed 100, 200, and 400 mg PHP per kilogram of basal diet, respectively. At 14, 21, 28 and 35 d of age, five chicks were randomly selected from each group to collect samples for index detection. The results showed that compared with Group A, there were significant reduction in average daily feed intake (ADFI) and feed-to-weight ratio (F/G) at 14, 21, and 28 d (P < 0.05), significant increase in average daily gain (ADG) at 21, 28 d (P < 0.05), significantly increased levels of total protein (TP), albumin (ALB), insulin (INS), thyroxine (T3), growth hormone (GH) at 14, 28 d (P < 0.05), significantly decreased levels of glucose (GLU), total cholesterol (TC), glucagon (GC), and triglyceride (TG) at 28 d in Group C (P < 0.05). There were significantly increased levels of TP, ALB at 14, 21 d (P < 0.05), significantly increased level of TP at 35 d (P < 0.05), significantly increased level of GH at 28 d (P < 0.05), significantly decreased levels of GLU, GC at 28 d (P < 0.05), significant reduction in F/G at 14, 21 d in Groups B and D (P < 0.05). Based on the above results, the livers from chicks in Groups A and C at 28 d were selected for transcriptome sequencing. The sequencing results showed that significantly differentially expressed genes (SDEGs) were enriched in growth and development, oxidative phosphorylation, the PPAR signaling pathway and the lipid metabolism pathway. All these results revealed that the addition of 200 mg/kg PHP in the diet promoted the growth and development, lipid metabolism and energy metabolism of chicks, inhibit inflammation and tumor development, and improve the function of the liver.
在这项研究中，通过给小鸡喂食饲料来研究 太子参多糖（PHP）对小鸡生长、发育和肝脏代谢的影响。选择了 400 只 7 日龄的固始公鸡，并将它们随机分为四组，分别标记为 A、B、C 和 D。A 组喂食基础日粮，B、C 和 D 组分别喂食每公斤基础日粮 100、200 和 400 毫克 PHP。在 14、21、28 和 35 日龄时，从每组中随机抽取 5 只小鸡收集样本进行指标检测。结果表明，与 A 组相比，14、21 和 28 日龄时平均日采食量（ADFI）和饲料增重比（F/G）显著降低（P<0.05），21、28 日龄时平均日增重（ADG）显著增加（P<0.05），14、28 日龄时总蛋白（TP）、白蛋白（ALB）、胰岛素（INS）、甲状腺素（T3）、生长激素（GH）水平显著升高（P<0.05），28 日龄时血糖（GLU）、总胆固醇（TC）、胰高血糖素（GC）、甘油三酯（TG）水平显著降低（P<0.05）。C 组在 14、21 日龄时 TP、ALB 水平显著升高（P<0.05），在 14、21 和 35 日龄时 TP 水平显著升高（P<0.05），在 28 日龄时 GH 水平显著升高（P<0.05），在 28 日龄时 GLU、GC 水平显著降低（P<0.05），在 14、21 日龄时 B、D 组 F/G 显著降低（P<0.05）。基于以上结果，选择 28 日龄时 A、C 组的小鸡肝脏进行转录组测序。测序结果表明，差异表达基因（DEGs）在生长发育、氧化磷酸化、PPAR 信号通路和脂质代谢通路中富集。所有这些结果表明，日粮中添加 200mg/kg PHP 可促进小鸡的生长发育、脂质代谢和能量代谢，抑制炎症和肿瘤发展，改善肝脏功能。
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