Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangdong Provincial Engineering Research Center for Ambient Mass Spectrometry, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), 100 Xianlie Middle Road, Guangzhou, 510070, China.
National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, Jishou University, Jishou, 427000, China.
Trop Anim Health Prod. 2022 Jan 11;54(1):45. doi: 10.1007/s11250-022-03044-7.
To investigate the potential of Ampelopsis grossedentata extract used as a feed additive, laying performance, egg quality, yolk cholesterol, plasma biochemical parameters, intestinal histology, and gut microbiota of hens (n = 60) were determined between basal diet (CK) and dietary supplementation with A. grossedentata extract (RT) for 11 weeks. The laying rate in RT group was 6.3 percentage points higher than in CK group together with feed conversion rate decreasing. Significant upregulation of immunoglobulin indexes and downregulation of lipid-related indexes in RT group were also found in comparison with CK group, suggesting that dietary supplementation with A. grossedentata extract benefited in immunity enhancing and blood-fat depressing. Meanwhile, the villus height in duodenum and villus height to crypt depth ratio in duodenum and jejunum of RT group were significantly higher than that of CK group, indicating that dietary supplementation with A. grossedentata extract facilitated nutrient adsorption via intestinal histology changing. Moreover, the richness, diversity, and composition of gut microbiota in RT group significantly altered with a comparison of CK group, including beneficial bacterium and pathogenic bacterium, revealing that dietary supplementation with A. grossedentata extract could modify gut microbiota communities to affect intestinal adsorption and pathogen invasion. In addition, the lipid metabolism-related insulin signing pathway was significantly enriched by gut microbiota in RT group, which were conducive to egg production elevation via facilitating blood lipid amelioration and insulin resistance alleviation. These results provided a basis for A. grossedentata extract served as a feed additive in the hen industry.
为了研究绞股蓝提取物作为饲料添加剂的潜力，我们在基础日粮（CK）中添加或不添加绞股蓝提取物（RT），连续喂养产蛋鸡 11 周，检测了母鸡的产蛋性能、蛋品质、蛋黄胆固醇、血浆生化参数、肠道组织学和肠道微生物群。与 CK 组相比，RT 组的产蛋率提高了 6.3 个百分点，饲料转化率降低。与 CK 组相比，RT 组的免疫球蛋白指标显著上调，脂质相关指标显著下调，提示日粮中添加绞股蓝提取物有利于增强免疫力和降低血脂。同时，RT 组十二指肠绒毛高度和十二指肠及空肠绒毛高度与隐窝深度比值显著高于 CK 组，表明日粮中添加绞股蓝提取物通过改变肠道组织学促进了营养物质的吸收。此外，与 CK 组相比，RT 组的肠道微生物丰富度、多样性和组成发生了显著变化，包括有益菌和病原菌，表明日粮中添加绞股蓝提取物可以改变肠道微生物群落，影响肠道吸收和病原体入侵。此外，RT 组肠道微生物群显著富集了与脂质代谢相关的胰岛素信号通路，这有利于通过改善血脂和缓解胰岛素抵抗来提高产蛋量。这些结果为绞股蓝提取物作为蛋鸡饲料添加剂提供了依据。
