Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300350, China.
Mar Drugs. 2024 May 9;22(5):216. doi: 10.3390/md22050216.
Microalgal lipids hold significant potential for the production of biodiesel and dietary supplements. To enhance their cost-effectiveness and commercial competitiveness, it is imperative to improve microalgal lipid productivity. Metabolic engineering that targets the key enzymes of the fatty acid synthesis pathway, along with transcription factor engineering, are effective strategies for improving lipid productivity in microalgae. This review provides a summary of the advancements made in the past 5 years in engineering the fatty acid biosynthetic pathway in eukaryotic microalgae. Furthermore, this review offers insights into transcriptional regulatory mechanisms and transcription factor engineering aimed at enhancing lipid production in eukaryotic microalgae. Finally, the review discusses the challenges and future perspectives associated with utilizing microalgae for the efficient production of lipids.
微藻油脂在生物柴油和膳食补充剂的生产方面具有重要的潜力。为了提高其成本效益和商业竞争力，提高微藻油脂的生产力至关重要。针对脂肪酸合成途径的关键酶的代谢工程以及转录因子工程是提高微藻油脂生产力的有效策略。本综述总结了过去 5 年在工程改造真核微藻脂肪酸生物合成途径方面的进展。此外，本综述还介绍了转录调控机制和转录因子工程，旨在提高真核微藻的油脂产量。最后，本文讨论了利用微藻高效生产油脂所面临的挑战和未来展望。
