Washburn L C, Hwa Sun T T, Crook J E, Byrd B L, Carlton J E, Hung Y W, Steplewski Z S
Int J Rad Appl Instrum B. 1986;13(4):453-6. doi: 10.1016/0883-2897(86)90024-3.
Monoclonal antibody 17-1A, which has specificity for colorectal carcinoma, was labeled with 90Y (10-20% radiolabeling yield). Tissue distribution studies in tumor-bearing nude mice were carried out. 90Y-labeled 17-1A showed good uptake in the SW 948 colon carcinoma cell line. However, 90Y-labeled A5C3, a monoclonal antihepatitis virus antibody studied as a control, showed similar uptake in this tumor. Neither antibody was taken up well by a WM-9 melanoma. It is believed that the loss of specificity observed is due to the low specific activity of the 90Y-labeled monoclonal antibody preparations used. This hypothesis is supported by radioimmunoassay data.
对结肠直肠癌具有特异性的单克隆抗体17 - 1A用90Y进行标记（放射性标记产率为10 - 20%）。在荷瘤裸鼠中进行了组织分布研究。90Y标记的17 - 1A在SW 948结肠癌细胞系中显示出良好的摄取。然而，作为对照研究的90Y标记的抗肝炎病毒单克隆抗体A5C3在该肿瘤中显示出相似的摄取。两种抗体在WM - 9黑色素瘤中摄取均不佳。据信观察到的特异性丧失是由于所使用的90Y标记单克隆抗体制剂的比活性较低。这一假设得到放射免疫分析数据的支持。
