Kitamura F, Shimada K, Suzuki T, Nishioka K
J Immunol Methods. 1985 Dec 27;85(2):363-70. doi: 10.1016/0022-1759(85)90145-0.
The inhibitory effect of methylprednisolone 21-succinate ester (MPS) on the activities of complement components in human serum was studied by incubating human serum with various concentrations of MPS at 37 degrees C for 30 min and then measuring the residual activity of each component in human serum. The formation of EAC1 and EAC14 by C1 and C4 respectively, were only weakly inhibited by MPS at a final concentration of 10 mg/ml. In contrast, the same concentration of MPS completely inhibited the capacity of C2, C3, C5 and C6-9 to induce respective succeeding intermediates. On the basis of these findings a simplified method was devised for the preparation of EAC14 intermediates using human serum pretreated with MPS.
通过将人血清与不同浓度的琥珀酸甲泼尼龙（MPS）在37℃孵育30分钟，然后测定人血清中各成分的残余活性，研究了琥珀酸甲泼尼龙（MPS）对人血清中补体成分活性的抑制作用。最终浓度为10mg/ml时，MPS仅对分别由C1和C4形成的EAC1和EAC14有微弱抑制作用。相比之下，相同浓度的MPS完全抑制了C2、C3、C5和C6-9诱导各自后续中间体的能力。基于这些发现，设计了一种使用经MPS预处理的人血清制备EAC14中间体的简化方法。
