黑猩猩中丁型肝炎病毒感染性的滴定

Titration of the infectivity of hepatitis D virus in chimpanzees.

作者信息

Ponzetto A, Hoyer B H, Popper H, Engle R, Purcell R H, Gerin J L

出版信息

J Infect Dis. 1987 Jan;155(1):72-8. doi: 10.1093/infdis/155.1.72.

DOI:10.1093/infdis/155.1.72

Abstract

The infectivity of hepatitis D virus (HDV) was evaluated by intravenous inoculation of chimpanzees. HDV was present in the inoculum at a titer of 10(11) chimpanzee infectious doses (CID). In contrast, the titer of hepatitis B virus (HBV) in the same inoculum was 10(6) CID. All HBV-infected chimpanzees inoculated with less than or equal to 10(-11) dilutions of the HDV-positive plasma were superinfected; an animal receiving a 10(-12) dilution did not develop markers of HDV replication in serum or liver. All HDV-infected chimpanzees had marked elevations of serum alanine aminotransferase activities. The incubation period from exposure to development of hepatitis was inversely related to the dose of HDV inoculum, although the severity and duration of hepatitis were independent of it. All animals recovered and rapidly developed antibody to hepatitis D antigen.

摘要

通过对黑猩猩进行静脉接种来评估丁型肝炎病毒（HDV）的传染性。接种物中HDV的滴度为10（11）黑猩猩感染剂量（CID）。相比之下，同一接种物中乙型肝炎病毒（HBV）的滴度为10（6）CID。所有接种了小于或等于HDV阳性血浆10（-11）稀释度的HBV感染黑猩猩均发生了重叠感染；接受10（-12）稀释度的一只动物血清或肝脏中未出现HDV复制标志物。所有HDV感染的黑猩猩血清丙氨酸转氨酶活性均显著升高。从接触到肝炎发生的潜伏期与HDV接种剂量呈负相关，尽管肝炎的严重程度和持续时间与之无关。所有动物均康复并迅速产生了抗丁型肝炎抗原抗体。