Latorre Joaquin Isper, Gonçalves Marcela Letícia Leal, González Carolina Stéfani Wince, Kassa Claudio Teruo, Rodriguez Mikelhy Silva, Tortamano Ana Carolina A C, Magalhães Fabiana Divina, Kato Ilka Tiemy, Bussadori Sandra Kalil, Prates Renato Araujo
Catholic University of Uruguay- Montevideo (UCU), Montevideo, Uruguay.
Biophotonics Medicine Postgraduate Program, Universidade Nove de Julho, São Paulo, Brazil.
Lasers Med Sci. 2025 Apr 14;40(1):189. doi: 10.1007/s10103-025-04434-7.
This study aims to analyze the effect of antimicrobial photodynamic therapy on Aggregatibacter actinomycetemcomitans using erythrosine as a photosensitizer and a blue light emitting-diode as a light source. Inoculum samples of A. actinomycetemcomitans with PBS were used in each of the groups, being the control group (C); light group (L) corresponding to light emitting-diode irradiation for 300 s; photosensitizing group (0) without irradiation; and the aPDT groups with different irradiation times (aPDT20) with 20s of irradiation; (aPDT40) with 40s of irradiation; (aPDT60) with 60s of irradiation; (aPDT180) with 180s; and (aPDT300) with 300s. Samples were used to determine colony forming units (CFU). Aliquots of 10 µL were plated through six serial dilutions on brain-heart infusion agar in Petri dishes. The plates were incubated at 37 °C for a period of up to 24-48 h under microaerophilic conditions to evaluate the total bacteria recovered. After this period, CFUs were counted, and the data was subjected to one-way analysis of variance. When aPDT was performed for 180 and 300 s, the mean log10 (CFU/ml) was equal to 0. In the aPDT60 group, a significant yet incomplete microbial reduction was observed. SEM images confirmed that membrane integrity was maintained, indicating that aPDT induced cellular alterations without causing membrane disruption. Antimicrobial photodynamic therapy employing erythrosine as a photosensitizer and blue light emitting-diode light-curing unit for composite resin polymerization used in dental practices demonstrated significant antimicrobial efficacy against A. actinomycetemcomitans, a principal pathogen in periodontitis, under the evaluated experimental conditions.
本研究旨在分析以赤藓红作为光敏剂、蓝光发光二极管作为光源的抗菌光动力疗法对伴放线聚集杆菌的作用。每组均使用含磷酸盐缓冲盐水（PBS）的伴放线聚集杆菌接种样本，分别为对照组（C）；光组（L），对应发光二极管照射300秒；光敏组（0），不进行照射；以及不同照射时间的抗菌光动力疗法组（aPDT20），照射20秒；（aPDT40），照射40秒；（aPDT60），照射60秒；（aPDT180），照射180秒；（aPDT300），照射300秒。使用样本测定菌落形成单位（CFU）。将10微升等分试样通过六次连续稀释接种在培养皿中的脑心浸液琼脂上。将平板在37°C下于微需氧条件下孵育长达24 - 48小时，以评估回收的总细菌数。在此时间段后，计数CFU，并对数据进行单因素方差分析。当进行180秒和300秒的抗菌光动力疗法时，平均log10（CFU/毫升）等于0。在aPDT60组中，观察到显著但不完全的微生物减少。扫描电子显微镜（SEM）图像证实膜完整性得以维持，表明抗菌光动力疗法诱导了细胞改变但未导致膜破坏。在评估的实验条件下，采用赤藓红作为光敏剂以及用于牙科实践中复合树脂聚合的蓝光发光二极管光固化单元的抗菌光动力疗法对牙周炎的主要病原体——伴放线聚集杆菌显示出显著的抗菌效果。
