Department of General Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
Department of Gastrointestinal Surgical Oncology, Clinical Oncology School of Fujian Medical University, Fuzhou, Fujian, China.
Cell Cycle. 2023 Oct;22(19):2161-2171. doi: 10.1080/15384101.2023.2278942. Epub 2023 Dec 5.
LncRNAs are widely linked to the complex development of gastric cancer, which is acknowledged worldwide as the third highest contributor to cancer-related deaths and the fifth most common form of cancer. The primary focus of this study is to examine the role of LncRNA PSMG3-AS1 in a group of individuals with gastric cancer. The results of our study indicate that PSMG3-AS1 is highly expressed in over 20 different types of cancer. Significantly, there was a clear association found between the expression of PSMG3-AS1 and a multitude of TMB and MSI tumors. PSMG3-AS1 exhibited significant upregulation in gastric cancer patients compared to healthy individuals within the gastric cancer cohort. The prognosis of gastric cancer patients is intrinsically associated with PSMG3-AS1, as confirmed by survival analysis and ROC curves. Furthermore, we created a disruption vector based on LncRNA PSMG3-AS1 and introduced it into AGS and MKN-45 cells, which are human gastric cancer cells. Significant decreases in the expression of the PSMG3-AS1 gene were noticed in both intervention groups compared to the NC group, reflecting the protein level expressions. Significantly, the proliferative and invasive capabilities of MKN-45 and AGS cells were notably reduced following transfection with PSMG3-AS1 siRNA. The results of our study indicate that disruption of the LncRNA PSMG3-AS1 gene may impact the CAV1/miR-451a signaling pathway, thereby leading to a reduction in the ability of gastric cancer cells to multiply and invade.
LncRNAs 与胃癌的复杂发展广泛相关，胃癌是全球第三大癌症相关死亡原因，也是第五种最常见的癌症。本研究的主要重点是研究 LncRNA PSMG3-AS1 在一组胃癌患者中的作用。我们的研究结果表明，PSMG3-AS1 在超过 20 种不同类型的癌症中高表达。重要的是，发现 PSMG3-AS1 的表达与多种 TMB 和 MSI 肿瘤之间存在明显关联。与胃癌队列中的健康个体相比，PSMG3-AS1 在胃癌患者中表达明显上调。生存分析和 ROC 曲线证实，PSMG3-AS1 与胃癌患者的预后密切相关。此外，我们基于 LncRNA PSMG3-AS1 创建了一个破坏载体，并将其引入人胃癌细胞 AGS 和 MKN-45 中。与 NC 组相比，两个干预组的 PSMG3-AS1 基因表达均显著下调，反映了蛋白水平的表达。重要的是，转染 PSMG3-AS1 siRNA 后，MKN-45 和 AGS 细胞的增殖和侵袭能力明显降低。我们的研究结果表明，破坏 LncRNA PSMG3-AS1 基因可能会影响 CAV1/miR-451a 信号通路，从而降低胃癌细胞的增殖和侵袭能力。
