State Key Laboratory of Supramolecular Structure and Material, Center for Supramolecular Chemical Biology, College of Chemistry, Jilin University, Changchun 130012, China.
Joint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun 130021, China.
ACS Appl Mater Interfaces. 2023 Nov 22;15(46):53283-53296. doi: 10.1021/acsami.3c12845. Epub 2023 Nov 10.
Probiotic therapy in infected wound healing is hindered by its low viability and colonization efficiency during treatments. Developing dressings that maintain metabolic activity and prevent the potential leakage of probiotics is imperative. Herein, a culture-delivery live probiotics hydrogel dressing is designed and synthesized, formed by gelatin modified with norbornene (GelNB) and sulfhydryl (GelSH), distributing ()-laden alginate microspheres (AlgMPs). GelNB-GelSH hydrogel (GelNBSH) incorporating AlgMPs embedding (GelNBSH-L) possesses bioprintability and efficient polymerization that can maintain the activity of in situ, promote its proliferation, and limit its leakage. Thereby, GelNBSH-L achieved a sustainable antimicrobial effect against both and (>90%). Above all, the results show that GelNBSH-L could ensure propitious viability and efficient antibacterial properties of probiotics, effectively inhibit the further development of bacterial infectious wounds and shorten the repair cycle, aiding in ameliorating future clinical probiotic biotherapy.
在感染性伤口愈合中，益生菌疗法受到其在治疗过程中低存活率和定植效率的阻碍。开发能够维持代谢活性并防止益生菌潜在泄漏的敷料是当务之急。本研究设计并合成了一种载菌-输送型活性益生菌水凝胶敷料，由用降冰片烯（GelNB）和巯基（GelSH）改性的明胶（GelSH）和（）-负载的海藻酸钠微球（AlgMPs）组成。含有 AlgMPs 嵌入物的 GelNB-GelSH 水凝胶（GelNBSH-L）具有生物打印性和高效聚合性，可原位维持 的活性，促进其增殖，并限制其泄漏。因此，GelNBSH-L 对 和 均具有可持续的抗菌作用（>90%）。综上所述，结果表明 GelNBSH-L 可以确保益生菌良好的存活率和高效的抗菌性能，有效抑制细菌感染性伤口的进一步发展，缩短修复周期，有助于改善未来临床益生菌生物治疗。
