Hospital Alemán [German Hospital], Argentina.
Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno [Norberto Quirno Centre for Medical Education and Clinical Research] (CEMIC), Argentina.
Rev Iberoam Micol. 2023 Apr-Jul;40(2-3):19-25. doi: 10.1016/j.riam.2021.01.006. Epub 2023 Nov 10.
Identification of dermatophytes is usually performed through morphological analyses. However, it may be hindered due to the discovery of new species and complexes and, with some isolates, by the absence of fructification. Matrix-assisted laser desorption/ionisation-time-of-flight mass spectrometry (MALDI-TOF MS) seems to be an option for improving identification.
To develop a database (DB) for the identification of dermatophytes with MALDI-TOF MS, including 32 isolates from the Red de Micología de la Ciudad Autónoma de Buenos Aires [Mycology Network of the Autonomous City of Buenos Aires] (RMCABA) and one reference isolate (RMCABA DB), and evaluate its performance when added to the DB from the supplier, Bruker (Bruker DB).
All the isolates in the RMCABA DB were identified based on morphology and sequencing. To evaluate the performance of the extended DB (Bruker DB plus RMCABA DB), 136 clinical isolates were included.
The percentages of identification at the species level increased from 45% to 88%, but the identification at the genus level decreased from 23% to 7%.
MALDI-TOF MS yielded better performance in the identification of dermatophytes after including the RMCABA DB, which encompassed local isolates.
通常通过形态分析来鉴定皮肤癣菌。然而，由于新物种和复合体的发现，以及某些分离株缺乏生殖结构，这可能会受到阻碍。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）似乎是提高鉴定能力的一种选择。
建立一个用于 MALDI-TOF MS 鉴定皮肤癣菌的数据库（DB），包括来自布宜诺斯艾利斯自治市真菌学网络（RMCABA）的 32 个分离株和一个参考分离株（RMCABA DB），并评估其在添加到供应商 Bruker 的 DB（Bruker DB）中的性能。
RMCABA DB 中的所有分离株均基于形态学和测序进行鉴定。为了评估扩展 DB（Bruker DB 加 RMCABA DB）的性能，纳入了 136 例临床分离株。
物种水平的鉴定百分比从 45%增加到 88%，但属水平的鉴定百分比从 23%下降到 7%。
在纳入 RMCABA DB 后，MALDI-TOF MS 对皮肤癣菌的鉴定性能更好，其中包括当地分离株。
