Department of Chemistry, School of Energy Technology, Pandit Deendayal Energy University, Raisan, Gujarat 382426, India.
Biological Sciences and Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gujarat 382355, India.
Int J Biol Macromol. 2024 Jan;255:128019. doi: 10.1016/j.ijbiomac.2023.128019. Epub 2023 Nov 10.
Worldwide, burn wounds are severe health issues prone to bacterial infections and challenging to treat with traditional wound dressings. Therefore, a highly desirable biological macromolecules-based wound dressing with good antioxidant, antibacterial, biocompatible, and a large surface area is required. Herein, aim to develop a biological macromolecules-based physically cross-linked gelatin/polyglyceryl stearate/graphene oxide (GPGO) hydrogel to treat burn wounds. Four sets of hydrogels were prepared by varying GO concentrations. FT-IR, FE-SEM, viscosity analysis, mechanical and thermal stability confirmed the successful preparation of hydrogels with desired properties. Further, β-carotene (0.5 mg/mL) was encapsulated in hydrogels to enhance the antioxidant activity, and a cumulative release as well as kinetics at pH 6.4 and 7.4 was performed. With an increase in GO concentration, hydrogels showed sustained release of β-carotene. Among all, GPGO-3 β hydrogel showed the highest antioxidant potency (57.75 %), hemocompatible (<5 %), cytocompatible (viable with NIH 3T3 cells), cell migration, proliferation, and in vitro wound healing. Also, GPGO-3 β hydrogel showed efficient antibacterial activity (%inhibition of 85.5 % and 80.2 % and zone of 11 mm and 9.8 mm against S. aureus and E. coli). These results demonstrated the ability of GPGO-3 β hydrogel as a promising candidate for burn wound healing applications.
在全球范围内，烧伤创面是严重的健康问题，容易受到细菌感染，并且用传统的创面敷料治疗具有挑战性。因此，需要一种具有良好抗氧化、抗菌、生物相容性和大表面积的理想生物大分子基创面敷料。在此，旨在开发一种基于生物大分子的物理交联明胶/聚甘油硬脂酸酯/氧化石墨烯（GPGO）水凝胶来治疗烧伤创面。通过改变 GO 浓度制备了四组水凝胶。FT-IR、FE-SEM、粘度分析、机械和热稳定性证实了具有所需性能的水凝胶的成功制备。此外，将β-胡萝卜素（0.5mg/mL）包封在水凝胶中以提高抗氧化活性，并在 pH 6.4 和 7.4 下进行累积释放和动力学研究。随着 GO 浓度的增加，水凝胶显示出β-胡萝卜素的持续释放。在所有水凝胶中，GPGO-3β水凝胶显示出最高的抗氧化能力（57.75%）、血液相容性（<5%）、细胞相容性（NIH 3T3 细胞存活）、细胞迁移、增殖和体外伤口愈合。此外，GPGO-3β水凝胶对金黄色葡萄球菌和大肠杆菌具有有效的抗菌活性（抑制率分别为 85.5%和 80.2%，抑菌圈分别为 11mm 和 9.8mm）。这些结果表明 GPGO-3β水凝胶作为一种有前途的烧伤创面愈合应用候选物的能力。
