Nishiwaki H, Iriyama K, Asami H, Kihata M, Hioki T, Asakawa T, Suzuki H
JPEN J Parenter Enteral Nutr. 1986 Nov-Dec;10(6):614-6. doi: 10.1177/0148607186010006614.
An experimental study was undertaken to study the influences of an infusion of lipid emulsion on phagocytosis of Kupffer's cells in septic rats. Sepsis was induced in 13 rats by ligating the cecum. Five of them received glucose as the sole nonprotein calorie (septic-glucose group), four of the rats received 25% of the nonprotein calorie with lipid emulsion, Intralipid (septic-lipid group), and the remaining four rats did not receive any intravenous solution and were allowed access to water (septic-fasted group). Another four rats which received neither intravenous solution nor ligation of the cecum served as the control group. The intravenous infusion was carried out for 72 hr. The phagocytotic activity of Kupffer's cells was determined by the ability to engulf latex particles with a size of 1.09 micron, in vitro. The phagocytotic activity was enhanced by the presence of sepsis but it was inhibited by starvation. The difference in the phagocytotic activity between the septic-glucose group and the septic-lipid group was not significant. These results suggest that, insofar as an in vitro study is concerned, a 72-hr infusion of lipid emulsion at a rate of 25% of the total nonprotein calorie does not influence the phagocytotic activity of cultured Kupffer's cell obtained from septic rats.
进行了一项实验研究，以探讨输注脂质乳剂对脓毒症大鼠库普弗细胞吞噬作用的影响。通过结扎盲肠在13只大鼠中诱导脓毒症。其中5只接受葡萄糖作为唯一的非蛋白热量来源（脓毒症-葡萄糖组），4只大鼠接受25%的非蛋白热量与脂质乳剂，英脱利匹特（脓毒症-脂质组），其余4只大鼠不接受任何静脉溶液，可自由饮水（脓毒症-禁食组）。另外4只既未接受静脉溶液也未结扎盲肠的大鼠作为对照组。静脉输注持续72小时。通过体外吞噬1.09微米大小乳胶颗粒的能力来测定库普弗细胞的吞噬活性。脓毒症会增强吞噬活性，但饥饿会抑制吞噬活性。脓毒症-葡萄糖组和脓毒症-脂质组之间的吞噬活性差异不显著。这些结果表明，就体外研究而言，以总非蛋白热量的25%的速率输注72小时脂质乳剂不会影响从脓毒症大鼠获得的培养库普弗细胞的吞噬活性。
