Ohtsuka K
Gan No Rinsho. 1986 Oct;32(13):1671-7.
Thermotolerance, or increased resistance of tissues to hyperthermia following a prior heat treatment, has been demonstrated not only in normal but also in tumor tissues. The kinetics of thermotolerance depends on the prior heating conditions, i.e. the greater the initial heat damage, the greater is the magnitude of thermotolerance and the longer is the time to reach maximum and the time to decay. Although thermotolerance develops less extensively in cultured cells in low pH medium and average tumor tissue pH is lower than the normal tissue pH, the magnitude of thermotolerance in tumor thus far examined is at least equal to that in the normal tissues. It is known that development of thermotolerance is well correlated with enhanced synthesis of heat shock proteins (HSPs). Recently, we purified HSP 70 (70 kilo dalton HSP) and prepared a rabbit antiserum against the HSP 70. In our preliminary experiments, HSP 70 could be detected and analyzed quantitatively not only in cultured cells but also in normal and tumor tissues by immunoblotting using the antiserum.
热耐受，即组织在先前热处理后对高温的抵抗力增强，不仅在正常组织中得到证实，在肿瘤组织中也得到了证实。热耐受的动力学取决于先前的加热条件，即初始热损伤越大，热耐受的程度就越大，达到最大值的时间和衰减时间就越长。尽管在低pH培养基中培养的细胞中热耐受的发展程度较低，且肿瘤组织的平均pH低于正常组织的pH，但迄今为止所检测的肿瘤中的热耐受程度至少与正常组织中的相等。已知热耐受的发展与热休克蛋白（HSPs）合成的增强密切相关。最近，我们纯化了HSP 70（70千道尔顿的HSP）并制备了针对HSP 70的兔抗血清。在我们的初步实验中，通过使用该抗血清进行免疫印迹，不仅可以在培养细胞中，还可以在正常组织和肿瘤组织中检测和定量分析HSP 70。
