Kaneko R, Hattori H, Hayashi Y, Tohnai I, Ueda M, Ohtsuka K
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
Radiat Res. 1995 Apr;142(1):91-7.
We have investigated the relationship between the synthesis of hsp-40, recently identified as a mammalian homologue of DnaJ protein, as well as hsp-70 and the development of thermotolerance in cells of the mouse squamous cell carcinoma line SCCVII. Thermotolerance as determined by clonogenic survival was induced not only by prior heating at 44 degrees C for 30 min but also by prior treatment with 100 microM sodium arsenite for 1 h and 5 micrograms/ml prostaglandin J2 for 4 h. These treatments concomitantly induced both hsp-70 (p72, inducible form) and hsp-40. The extent of thermotolerance correlated well with the relative amount of hsp-70 and hsp-40. These results suggest that hsp-40 is a good predictor of thermotolerance in addition to hsp-70.
我们已经研究了热休克蛋白40（hsp-40，最近被鉴定为DnaJ蛋白的哺乳动物同源物）以及热休克蛋白70（hsp-70）的合成与小鼠鳞状细胞癌系SCCVII细胞耐热性发展之间的关系。通过克隆形成存活率测定的耐热性不仅可由之前在44℃加热30分钟诱导产生，还可由之前用100微摩尔/升亚砷酸钠处理1小时以及用5微克/毫升前列腺素J2处理4小时诱导产生。这些处理同时诱导了hsp-70（p72，可诱导形式）和hsp-40的产生。耐热性的程度与hsp-70和hsp-40的相对量密切相关。这些结果表明，除了hsp-70之外，hsp-40也是耐热性的一个良好预测指标。
