Jiménez-Nevárez Yazmín B, Montes-Avila Julio, Angulo-Escalante Miguel Angel, Nolasco-Quintana Ninfa Yaret, González Christen Judith, Hurtado-Díaz Israel, Quintana-Obregón Eber Addí, Heredia J Basilio, Valdez-Torres José Benigno, Alvarez Laura
Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Eldorado km 5.5, Campo El Diez, Culiacán 80110, Mexico.
Programa de Posgrado en Ciencias Biomédicas, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria s/n, Culiacán 80010, Mexico.
Plants (Basel). 2023 Nov 6;12(21):3780. doi: 10.3390/plants12213780.
Medicines for chronic inflammation can cause gastric ulcers and hepatic and renal issues. An alternative treatment for chronic inflammation is that of natural bioactive compounds, which present low side effects. Extracts of (Ortega) Müll. Arg. have been evaluated for their cytotoxicity and anti-inflammatory activity; however, testing pure compounds would be of greater interest. Campesteryl palmitate, n-heptyl ferulate, palmitic acid, and a mixture of sterols, i.e., brassicasterol, campesterol, β-sitosterol, and stigmasterol, were obtained from an ethyl acetate extract from (Ortega) Müll. Arg. bark using column chromatography. The toxicity and in vitro anti-inflammatory activities were evaluated using RAW 264.7 murine macrophage cells. None of the products assessed exhibited toxicity. The sterol mixture exhibited greater anti-inflammatory activity than the positive control, and nitric oxide (NO) inhibition percentages were 37.97% and 41.68% at 22.5 μg/mL and 30 μg/mL, respectively. In addition, n-heptyl ferulate decreased NO by 30.61% at 30 μg/mL, while campesteryl palmitate did not show anti-inflammatory activity greater than the positive control. The mixture and n-heptyl ferulate showed NO inhibition; hence, we may conclude that these compounds have anti-inflammatory potential. Additionally, further research and clinical trials are needed to fully explore the therapeutic potential of these bioactive compounds and their efficacy in treating chronic inflammation.
用于慢性炎症的药物可能会导致胃溃疡以及肝脏和肾脏问题。慢性炎症的一种替代治疗方法是使用天然生物活性化合物，其副作用较小。已对（奥尔特加）穆勒·阿格的提取物的细胞毒性和抗炎活性进行了评估；然而，测试纯化合物会更有意义。棕榈酸 campesteryl酯、阿魏酸正庚酯、棕榈酸以及一种甾醇混合物，即油菜甾醇、菜油甾醇、β-谷甾醇和豆甾醇，是通过柱色谱法从（奥尔特加）穆勒·阿格树皮的乙酸乙酯提取物中获得的。使用RAW 264.7小鼠巨噬细胞评估了其毒性和体外抗炎活性。所评估的产品均未表现出毒性。甾醇混合物表现出比阳性对照更强的抗炎活性，在22.5μg/mL和30μg/mL时，一氧化氮（NO）抑制率分别为37.97%和41.68%。此外，阿魏酸正庚酯在30μg/mL时使NO降低了30.61%，而棕榈酸campesteryl酯未表现出比阳性对照更强的抗炎活性。该混合物和阿魏酸正庚酯表现出NO抑制作用；因此，我们可以得出结论，这些化合物具有抗炎潜力。此外，需要进一步的研究和临床试验来充分探索这些生物活性化合物的治疗潜力及其在治疗慢性炎症方面的疗效。
