Tuchmann A, Fischer P L, Bauer P, Plenk H, Braun O, Dinstl K
Langenbecks Arch Chir. 1986;368(2):125-36. doi: 10.1007/BF01273851.
The study hereby submitted has been designed to show whether there are any differences between using a conventional knife or a CO2-laser when excising and grafting tumors in animal experiments. The animals were C57Bl/6 mice, the tumor was the Lewis lung carcinoma. Eight days after tumor inoculation into the subcutaneous layer of the back, 213 mice were treated by tumor excision either with a conventional knife or a CO2-laser. 86 excised tumors were cut and the tumor surface was swabbed into the subcutaneous layer on the nape of 86 tumor-free mice. Survival times of the laser-operated animals were insignificantly longer. However, small tumors showed markedly longer survival times. The interval without recurrence was longer for all tumor sizes when the laser was used (p less than 0.005). The laser method yielded lower growth rates when the tumor surface was swabbed into tumor-free mice (p less than 0.0001). Histological and cytological tests of the laser-excised and -swabbed specimen demonstrated a high rate of cell destruction. Therefore, the CO2-laser seems to have some importance in cases where the incision is made close to the tumor or where the tumor surface may be lesioned.
本研究旨在表明在动物实验中切除和移植肿瘤时，使用传统手术刀或二氧化碳激光是否存在差异。实验动物为C57Bl/6小鼠，肿瘤为Lewis肺癌。在将肿瘤接种到背部皮下层8天后，213只小鼠接受了用传统手术刀或二氧化碳激光进行的肿瘤切除治疗。将86个切除的肿瘤切开，并将肿瘤表面擦拭物植入86只无肿瘤小鼠颈部的皮下层。接受激光手术的动物存活时间略长。然而，小肿瘤的存活时间明显更长。使用激光时，所有肿瘤大小的无复发间隔时间都更长（p<0.005）。当将肿瘤表面擦拭物植入无肿瘤小鼠体内时，激光方法产生的生长速率较低（p<0.0001）。对激光切除和擦拭的标本进行组织学和细胞学检查表明细胞破坏率很高。因此，在切口靠近肿瘤或肿瘤表面可能受损的情况下，二氧化碳激光似乎具有一定重要性。
