Oosterhuis J W, Verschueren R C, Eibergen R, Oldhoff J
Cancer. 1982 Jan 1;49(1):61-7. doi: 10.1002/1097-0142(19820101)49:1<61::aid-cncr2820490114>3.0.co;2-n.
The viability of cells present in waste products originating during CO2-laser evaporation of Cloudman S91 mouse melanomas was investigated. Cytologic smears showed mainly carbonized particles and thermally damaged cells but some morphologically intact cells as well. Viability was tested with the trypan blue test, by in vitro culture and intramuscular and intraperitoneal inoculations in syngeneic mice. No dye-excluding cells were found in the trypan blue test. Growth was noted neither in vitro nor in vivo in the 127 mice, intramuscularly or intraperitoneally injected with 10(5) x 10(5)/10 microliter smoke or debris particles, and killed four weeks after inoculation. Viable tumor cells, derived from a Cloudman melanoma cell line and added to the smoke and debris suspensions, remained viable. Thus, a toxic influence of smoke and debris particles in viable tumor cells, which would make the viability tests meaningless, was excluded. It is highly unlikely that viable tumor cells do occur in the waste products of CO2-laser tumor vaporization.
对二氧化碳激光蒸发Cloudman S91小鼠黑色素瘤过程中产生的废品中的细胞活力进行了研究。细胞学涂片主要显示碳化颗粒和热损伤细胞，但也有一些形态完整的细胞。通过台盼蓝试验、体外培养以及在同基因小鼠体内进行肌肉注射和腹腔注射来测试细胞活力。在台盼蓝试验中未发现拒染细胞。在127只小鼠中，无论是肌肉注射还是腹腔注射10(5)×10(5)/10微升烟雾或碎片颗粒，接种四周后处死，均未观察到体外或体内生长。源自Cloudman黑色素瘤细胞系并添加到烟雾和碎片悬浮液中的活肿瘤细胞仍保持活力。因此，排除了烟雾和碎片颗粒对活肿瘤细胞的毒性影响，这种毒性影响会使活力测试变得毫无意义。二氧化碳激光肿瘤汽化的废品中极不可能存在活肿瘤细胞。
