Jia Hongchang, Liang Xin, Zhang Lixin, Zhang Jinmei, Sapey Enoch, Liu Xianyuan, Sun Yanhui, Sun Shi, Yan Hongrui, Lu Wencheng, Han Tianfu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Heihe Branch, Heilongjiang Academy of Agricultural Sciences, Heihe, China.
Front Plant Sci. 2022 Jun 30;13:920522. doi: 10.3389/fpls.2022.920522. eCollection 2022.
Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6-10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at -196 or -80°C. culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
保存有活力的花粉对于育种者维持理想种质以用于未来的自交繁殖具有重大意义。花粉的超低温保存是长期储存植物种质资源的一种有效且安全的方法。在本研究中，我们改进了大豆花粉超低温保存的方法。在大豆植株盛花期的上午6 - 10点采集初开阶段的大豆花，脱水10小时，然后冷冻并储存在-196或-80°C。培养实验表明，保存花粉的活力仍高达约90%。进行了非生长季（本地黑河）和异地（北京，从黑河长途快递后）杂交验证，发现保存花粉和新鲜花粉之间的真杂种率没有显著差异。大豆花粉超低温保存技术可以打破大豆杂交的时空限制，促进大豆工程育种的发展。
