Wang Mengya, Ji Qiushuang, Lai Bin, Liu Yirong, Mei Kunrong
Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China.
Comput Struct Biotechnol J. 2023 Oct 27;21:5358-5371. doi: 10.1016/j.csbj.2023.10.046. eCollection 2023.
Natural products synthesized by plants have substantial industrial and medicinal values and are therefore attracting increasing interest in various related industries. Among the key enzyme families involved in the biosynthesis of natural products, uridine diphosphate-dependent glycosyltransferases (UGTs) play a crucial role in plants. In recent years, significant efforts have been made to elucidate the catalytic mechanisms and substrate recognition of plant UGTs and to improve them for desired functions. In this review, we presented a comprehensive overview of all currently published structures of plant UGTs, along with in-depth analyses of the corresponding catalytic and substrate recognition mechanisms. In addition, we summarized and evaluated the protein engineering strategies applied to improve the catalytic activities of plant UGTs, with a particular focus on high-throughput screening methods. The primary objective of this review is to provide readers with a comprehensive understanding of plant UGTs and to serve as a valuable reference for the latest techniques used to improve their activities.
植物合成的天然产物具有重要的工业和药用价值，因此在各个相关行业中受到越来越多的关注。在参与天然产物生物合成的关键酶家族中，尿苷二磷酸依赖性糖基转移酶（UGTs）在植物中起着至关重要的作用。近年来，人们为阐明植物UGTs的催化机制和底物识别并对其进行改良以实现所需功能付出了巨大努力。在本综述中，我们全面概述了目前已发表的所有植物UGTs结构，并深入分析了相应的催化和底物识别机制。此外，我们总结并评估了用于提高植物UGTs催化活性的蛋白质工程策略，特别关注高通量筛选方法。本综述的主要目的是让读者全面了解植物UGTs，并为用于提高其活性的最新技术提供有价值的参考。
