Kendrick K M, Keverne E B, Baldwin B A, Sharman D F
Neuroendocrinology. 1986;44(2):149-56. doi: 10.1159/000124638.
Acetylcholinesterase (AChE) activity, and concentrations of monoamines, monoamine metabolites and oxytocin (OT) were measured in the cerebrospinal fluid (CSF) of sheep during late pregnancy, labour, parturition, vaginocervical stimulation, lamb separation and suckling. Concentrations of AChE, 4-hydroxy-3 methoxyphenylethan-1,2-diol (MHPG) and OT were significantly elevated during labour and parturition. OT levels were also significantly raised in cycling ewes given vaginocervical stimulation. Separation of the ewes from their lambs (0.5-2 h) caused significant increases in AChE and MHPG, but not in OT. During suckling, following reunion of the ewes and lambs, concentrations of AChE and OT were significantly raised. The dopamine metabolites 3,4-dihydroxyphenylacetic acid and homovanillic acid were significantly lower in CSF during late pregnancy than during parturition and post-partum. Intravenous injections of OT which produced high circulating levels of this hormone in plasma produced significant, but very small, increases in concentrations of OT in the CSF. Our results show that in the sheep, labour, parturition, suckling and vaginocervical stimulation provoke a release of OT in the brain similar to that in the peripheral circulation. Changes in CSF levels of AChE and MHPG during labour, parturition and lamb separation, but not during vaginocervical stimulation, may be related to stress or hypertension.
在妊娠后期、分娩期、产后、阴道宫颈刺激、羔羊分离和哺乳期间，测量了绵羊脑脊液（CSF）中的乙酰胆碱酯酶（AChE）活性、单胺、单胺代谢物和催产素（OT）的浓度。在分娩和产后期间，AChE、4-羟基-3-甲氧基苯乙醇-1,2-二醇（MHPG）和OT的浓度显著升高。给予阴道宫颈刺激的发情母羊的OT水平也显著升高。母羊与羔羊分离（0.5 - 2小时）导致AChE和MHPG显著增加，但OT没有增加。在哺乳期间，母羊和羔羊 reunion后，AChE和OT的浓度显著升高。妊娠后期脑脊液中的多巴胺代谢物3,4-二羟基苯乙酸和高香草酸比分娩期和产后显著降低。静脉注射OT使血浆中该激素的循环水平升高，导致脑脊液中OT浓度显著但非常小的增加。我们的结果表明，在绵羊中，分娩、产后、哺乳和阴道宫颈刺激会引起大脑中OT的释放，类似于外周循环中的释放。在分娩、产后和羔羊分离期间脑脊液中AChE和MHPG水平的变化，但在阴道宫颈刺激期间没有变化，可能与应激或高血压有关。
