适体的体外筛选及其应用。
In vitro selection of aptamers and their applications.
作者信息
DeRosa M C, Lin A, Mallikaratchy P, McConnell E M, McKeague M, Patel R, Shigdar S
机构信息
Department of Chemistry and Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1T2S2.
Department of Chemistry, Faculty of Sciences, McGill University, Montreal, QC, Canada, H3A 0B8.
出版信息
Nat Rev Methods Primers. 2023;3. doi: 10.1038/s43586-023-00247-6. Epub 2023 Jul 20.
Abstract
The introduction of the in-vitro evolution method known as SELEX (Systematic Evolution of Ligands by Exponential enrichment) more than 30 years ago led to the conception of versatile synthetic receptors known as aptamers. Offering many benefits such as low cost, high stability and flexibility, aptamers have sparked innovation in molecular diagnostics, enabled advances in synthetic biology and have facilitated new therapeutic approaches. The SELEX method itself is inherently adaptable and offers near limitless possibilities in yielding functional nucleic acid ligands. This Primer serves to provide guidance on experimental design and highlight new growth areas for this impactful technology.
摘要
30多年前，体外进化方法SELEX（指数富集配体系统进化）的引入催生了被称为适配体的多功能合成受体的概念。适配体具有低成本、高稳定性和灵活性等诸多优点，激发了分子诊断领域的创新，推动了合成生物学的发展，并促进了新的治疗方法的出现。SELEX方法本身具有内在的适应性，在产生功能性核酸配体方面提供了几乎无限的可能性。本入门指南旨在为实验设计提供指导，并突出这一有影响力的技术的新增长领域。
相似文献
1
In vitro selection of aptamers and their applications.
Nat Rev Methods Primers. 2023;3. doi: 10.1038/s43586-023-00247-6. Epub 2023 Jul 20.
2
[Advancements in complex target systematic evolution of ligands by exponential enrichment].
Se Pu. 2018 Oct 8;36(10):947-951. doi: 10.3724/SP.J.1123.2018.05019.
3
Aptamer Technologies in Neuroscience, Neuro-Diagnostics and Neuro-Medicine Development.
Molecules. 2024 Mar 2;29(5):1124. doi: 10.3390/molecules29051124.
4
Evolution of multi-functional capillary electrophoresis for high-efficiency selection of aptamers.
Biotechnol Adv. 2019 Dec;37(8):107432. doi: 10.1016/j.biotechadv.2019.107432. Epub 2019 Aug 19.
5
Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches.
Trends Biotechnol. 2022 May;40(5):549-563. doi: 10.1016/j.tibtech.2021.09.011. Epub 2021 Oct 28.
6
Primer-free aptamer selection using a random DNA library.
J Vis Exp. 2010 Jul 26(41):2039. doi: 10.3791/2039.
7
Systematic Study of Selection Stringency Reveals How To Enrich High-Affinity Aptamers.
J Am Chem Soc. 2023 Jan 11;145(1):194-206. doi: 10.1021/jacs.2c09522. Epub 2022 Dec 27.
8
In vitro RNA SELEX for the generation of chemically-optimized therapeutic RNA drugs.
Methods. 2016 Jul 1;103:167-74. doi: 10.1016/j.ymeth.2016.03.003. Epub 2016 Mar 10.
9
Recent progress of SELEX methods for screening nucleic acid aptamers.
Talanta. 2024 Jan 1;266(Pt 1):124998. doi: 10.1016/j.talanta.2023.124998. Epub 2023 Jul 26.
10
Strategies for Improving Aptamer Screening Efficiency: Library Design, Awareness Innovation, and Instrument Assistance.
Crit Rev Anal Chem. 2024 Oct 8:1-21. doi: 10.1080/10408347.2024.2411244.
引用本文的文献
1
Design and development of an aptamer targeting C-type lectin-like molecule-1 as a biomarker for acute myeloid leukemia: a SELEX approach.
Front Bioeng Biotechnol. 2025 Aug 1;13:1601453. doi: 10.3389/fbioe.2025.1601453. eCollection 2025.
2
Droplet microfluidics for single-cell studies: a frontier in ecological understanding of microbiomes.
FEMS Microbiol Rev. 2025 Jan 14;49. doi: 10.1093/femsre/fuaf032.
3
Advancements and Prospects in DNA-Based Bioanalytical Technology for Environmental Toxicant Detection.
JACS Au. 2025 Jun 2;5(6):2443-2462. doi: 10.1021/jacsau.5c00398. eCollection 2025 Jun 23.
4
Recent Advances in Aptamer-Based Point-of-Care Testing.
Sensors (Basel). 2025 Jun 6;25(12):3587. doi: 10.3390/s25123587.
5
Advantages, applications, and future directions of aptamer SELEX: A review.
Mol Ther Nucleic Acids. 2025 May 26;36(3):102575. doi: 10.1016/j.omtn.2025.102575. eCollection 2025 Sep 9.
6
Beyond traditional diagnosis: aptamer-based microRNA detection for the early diagnosis of periodontitis.
Mol Biol Rep. 2025 Jun 16;52(1):604. doi: 10.1007/s11033-025-10676-z.
7
Nanomedicine in Cancer Therapeutics: Current Perspectives from Bench to Bedside.
Mol Cancer. 2025 Jun 9;24(1):169. doi: 10.1186/s12943-025-02368-w.
8
Evolution of multivalent supramolecular assemblies of aptamers with target-defined spatial organization.
Nat Nanotechnol. 2025 Jun 6. doi: 10.1038/s41565-025-01939-8.
9
Novel Aptamers Targeting Sclerostin Loop3 Improve Skeletal and Muscle Properties Without Adverse Cardiovascular Effects in Orchiectomized Mice.
J Cachexia Sarcopenia Muscle. 2025 Jun;16(3):e13831. doi: 10.1002/jcsm.13831.
10
Aptamers: Design, Theory, and Applications to Diagnosis and Therapy for Diseases.
MedComm (2020). 2025 May 19;6(5):e70180. doi: 10.1002/mco2.70180. eCollection 2025 May.
本文引用的文献
1
Generative aptamer discovery using RaptGen.
Nat Comput Sci. 2022 Jun;2(6):378-386. doi: 10.1038/s43588-022-00249-6. Epub 2022 Jun 2.
2
Development of DNA aptamers for visualization of glial brain tumors and detection of circulating tumor cells.
Mol Ther Nucleic Acids. 2023 Mar 24;32:267-288. doi: 10.1016/j.omtn.2023.03.015. eCollection 2023 Jun 13.
3
An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations.
Micromachines (Basel). 2023 Feb 16;14(2):459. doi: 10.3390/mi14020459.
4
Design and Prediction of Aptamers Assisted by In Silico Methods.
Biomedicines. 2023 Jan 26;11(2):356. doi: 10.3390/biomedicines11020356.
5
Discovery of DNA aptamers targeting SARS-CoV-2 nucleocapsid protein and protein-binding epitopes for label-free COVID-19 diagnostics.
Mol Ther Nucleic Acids. 2023 Mar 14;31:731-743. doi: 10.1016/j.omtn.2023.02.010. Epub 2023 Feb 14.
6
Aptamer-based rapid diagnosis for point-of-care application.
Microfluid Nanofluidics. 2023;27(2):15. doi: 10.1007/s10404-022-02622-3. Epub 2023 Jan 18.
7
A novel biosensing platform for detection of glaucoma biomarker GDF15 via an integrated BLI-ELASA strategy.
Biomaterials. 2023 Mar;294:121997. doi: 10.1016/j.biomaterials.2023.121997. Epub 2023 Jan 9.
8
Machine Learning Directed Aptamer Search from Conserved Primary Sequences and Secondary Structures.
ACS Synth Biol. 2023 Jan 20;12(1):186-195. doi: 10.1021/acssynbio.2c00462. Epub 2023 Jan 3.
9
Diversifying Design of Nucleic Acid Aptamers Using Unsupervised Machine Learning.
J Phys Chem B. 2023 Jan 12;127(1):62-68. doi: 10.1021/acs.jpcb.2c05660. Epub 2022 Dec 27.
10
Diagnostic accuracy of vaccine and vaccine excipient testing in the setting of allergic reactions to COVID-19 vaccines: A systematic review and meta-analysis.
Allergy. 2023 Jan;78(1):71-83. doi: 10.1111/all.15571. Epub 2022 Nov 23.
Zotero 插件