Luo Senlin, Zhang Xiaotian, Zhang Yuzhuo, Qu Xiaofeng, Sun Qiru, Peng Tianhuan, Yuan Quan
Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China.
Sensors (Basel). 2025 Jun 6;25(12):3587. doi: 10.3390/s25123587.
Aptamers refer to a class of oligonucleotide probes that have demonstrated remarkable capabilities beyond mere genetic coding, including the unique ability to recognize and selectively bind to specific molecular targets. Numerous advantages, including accessibility for targeting a diverse array of molecules and compatibility with different signal amplification and transduction elements, underscore the application of aptamers for delivering rapid and accurate diagnostic tests at the point of care. This review provides a comprehensive summary of the recent advances in aptamer-based point-of-care testing, especially highlighting the innovative applications of aptamers in colorimetric sensors, lateral flow assays, fluorescent biosensors, and electrochemical biosensors. Additionally, current challenges in this burgeoning field and forward-looking perspectives for aptamer-based point-of-care testing are discussed.
适体是指一类寡核苷酸探针，它们已展现出超越单纯基因编码的显著能力，包括识别并选择性结合特定分子靶标的独特能力。适体具有诸多优势，例如能够针对多种不同分子，且与不同的信号放大和转导元件兼容，这些优势突出了其在即时检测中实现快速准确诊断测试的应用价值。本综述全面总结了基于适体的即时检测的最新进展，特别强调了适体在比色传感器、侧向流动分析、荧光生物传感器和电化学生物传感器中的创新应用。此外，还讨论了这一新兴领域当前面临的挑战以及基于适体的即时检测的前瞻性展望。
