Du Fangfang, Gao Yuan, Zhang Xibo, Wang Li-Li
School of Pharmaceutical Science, Postdoctoral Research Station of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China.
ACS Omega. 2023 Oct 23;8(44):41383-41390. doi: 10.1021/acsomega.3c05093. eCollection 2023 Nov 7.
Doxorubicin (DOX) is a potent anticancer drug, but it has side effects on normal tissues, particularly myocardial cells. Therefore, it is crucial to detect the DOX concentration in body fluids for effective clinical treatment. In this work, N,Bi-codoped CDs (Bi,N-CDs) were synthesized through a one-step hydrothermal method to carbonize the raw materials of 2,4-dinitroaniline and bismuth nitrate. The resulting Bi,N-CDs showed a reduced emission at 490 nm and an enhanced emission at 590 nm in the presence of DOX. The ratio of fluorescence (FL) intensity (F/F) was found to be a reliable indicator of DOX concentration, ranging from 0.05 to 30 μM and 40-200 μM, with detection limits (LOD) of 34 and 24 nM, respectively. A ratiometric fluorescence nanoprobe was established for highly selective and sensitive detection of DOX using a specific electrostatic interaction and inner filter effect between Bi,N-CDs and DOX. Meanwhile, Bi,N-CDs exhibited a distinct color change ranging from yellow to orange-red when exposed to DOX, allowing for a colorimetric method to measure DOX levels in the range of 0.05-30 μM, with a detection limit of 169 nM. The probe was triumphantly used to monitor DOX in actual samples via a dual-mode optical sensing strategy. This study contributes to the development of heteroatom-doped CDs and expands their potential applications for detecting biological samples.
阿霉素（DOX）是一种强效抗癌药物，但它对正常组织，尤其是心肌细胞有副作用。因此，检测体液中的DOX浓度对于有效的临床治疗至关重要。在这项工作中，通过一步水热法合成了N、Bi共掺杂的碳点（Bi，N-CDs），以碳化2,4-二硝基苯胺和硝酸铋的原料。在DOX存在的情况下，所得的Bi，N-CDs在490nm处发射减弱，在590nm处发射增强。荧光（FL）强度比（F/F）被发现是DOX浓度的可靠指标，浓度范围为0.05至30μM和40 - 200μM，检测限（LOD）分别为34和24 nM。利用Bi，N-CDs与DOX之间的特定静电相互作用和内滤效应，建立了一种用于高选择性和灵敏检测DOX的比率荧光纳米探针。同时，Bi，N-CDs在暴露于DOX时呈现出从黄色到橙红色的明显颜色变化，从而实现了一种比色法来测量0.05 - 30μM范围内的DOX水平，检测限为169 nM。该探针通过双模式光学传感策略成功用于监测实际样品中的DOX。这项研究有助于杂原子掺杂碳点的发展，并扩展了它们在检测生物样品方面的潜在应用。
