Key Laboratory for Biomechanics and Mechanobiology (Beihang University) of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, PR China.
Peking University Third Hospital, Department of Orthopaedics, PR China; Peking University Third Hospital, Engineering Research Center of Bone and Joint Precision Medicine, PR China.
Int J Biol Macromol. 2024 Jan;256(Pt 1):128031. doi: 10.1016/j.ijbiomac.2023.128031. Epub 2023 Nov 14.
Silks fibroin can be chemically modified through amino acid side chains to obtain methacrylated silk (Sil-MA). Sil-MA could be processed into a variety of scaffold forms and combine synergistically with other biomaterials to form composites vehicle. The advent of Sil-MA material has enabled impressive progress in the development of various scaffolds based on Sil-MA type to imitate the structural and functional characteristics of natural tissues. This review highlights the reasonable design and bio-fabrication strategies of diverse Sil-MA-based tissue constructs for regenerative medicine. First, we elucidate modification methodology and characteristics of Sil-MA. Next, we describe characteristics of Sil-MA hydrogels, and focus on the design approaches and formation of different types of Sil-MA-based hydrogels. Thereafter, we present an overview of the recent advances in the application of Sil-MA based scaffolds for regenerative medicine, including detailed strategies for the engineering methods and materials used. Finally, we summarize the current research progress and future directions of Sil-MA in regenerative medicine. This review not only delineates the representative design strategies and their application in regenerative medicine, but also provides new direction in the fabrication of biomaterial constructs for the clinical translation in order to stimulate the future development of implants.
丝素蛋白可以通过氨基酸侧链进行化学修饰，得到甲基丙烯酰化丝素（Sil-MA）。Sil-MA 可以加工成各种支架形式，并与其他生物材料协同结合，形成复合材料载体。Sil-MA 材料的出现使得基于 Sil-MA 型的各种支架的开发取得了令人瞩目的进展，这些支架可以模仿天然组织的结构和功能特性。本综述重点介绍了基于 Sil-MA 的组织构建体的合理设计和生物制造策略，用于再生医学。首先，我们阐明了 Sil-MA 的修饰方法和特性。接下来，我们描述了 Sil-MA 水凝胶的特性，并重点介绍了不同类型的基于 Sil-MA 的水凝胶的设计方法和形成。此后，我们概述了基于 Sil-MA 的支架在再生医学中的最新应用进展，包括用于工程方法和材料的详细策略。最后，我们总结了 Sil-MA 在再生医学中的当前研究进展和未来方向。本综述不仅阐述了代表性的设计策略及其在再生医学中的应用，还为生物材料构建体的制造提供了新的方向，以促进其在临床上的转化，从而激发植入物的未来发展。
