Chae Suhun, Cho Dong-Woo
Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Gyeongsangbuk-do, Pohang 37673, South Korea; EDmicBio Inc., 111 Hoegi-ro, Dongdaemun-gu, Seoul 02445, South Korea.
Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Gyeongsangbuk-do, Pohang 37673, South Korea; Institute for Convergence Research and Education in Advanced Technology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
Acta Biomater. 2023 Jan 15;156:4-20. doi: 10.1016/j.actbio.2022.08.004. Epub 2022 Aug 10.
The advent of three-dimensional (3D) bioprinting has enabled impressive progress in the development of 3D cellular constructs to mimic the structural and functional characteristics of natural tissues. Bioprinting has considerable translational potential in tissue engineering and regenerative medicine. This review highlights the rational design and biofabrication strategies of diverse 3D bioprinted tissue constructs for orthopedic tissue engineering applications. First, we elucidate the fundamentals of 3D bioprinting techniques and biomaterial inks and discuss the basic design principles of bioprinted tissue constructs. Next, we describe the rationale and key considerations in 3D bioprinting of tissues in many different aspects. Thereafter, we outline the recent advances in 3D bioprinting technology for orthopedic tissue engineering applications, along with detailed strategies of the engineering methods and materials used, and discuss the possibilities and limitations of different 3D bioprinted tissue products. Finally, we summarize the current challenges and future directions of 3D bioprinting technology in orthopedic tissue engineering and regenerative medicine. This review not only delineates the representative 3D bioprinting strategies and their tissue engineering applications, but also provides new insights for the clinical translation of 3D bioprinted tissues to aid in prompting the future development of orthopedic implants. STATEMENT OF SIGNIFICANCE: 3D bioprinting has driven major innovations in the field of tissue engineering and regenerative medicine; aiming to develop a functional viable tissue construct that provides an alternative regenerative therapy for musculoskeletal tissue regeneration. 3D bioprinting-based biofabrication strategies could open new clinical possibilities for creating equivalent tissue substitutes with the ability to customize them to meet patient demands. In this review, we summarize the significance and recent advances in 3D bioprinting technology and advanced bioinks. We highlight the rationale for biofabrication strategies using 3D bioprinting for orthopedic tissue engineering applications. Furthermore, we offer ample perspective and new insights into the current challenges and future direction of orthopedic bioprinting translation research.
三维（3D）生物打印技术的出现，使得在开发用于模拟天然组织结构和功能特征的3D细胞构建体方面取得了令人瞩目的进展。生物打印在组织工程和再生医学领域具有相当大的转化潜力。本综述重点介绍了用于骨科组织工程应用的各种3D生物打印组织构建体的合理设计和生物制造策略。首先，我们阐明3D生物打印技术和生物材料墨水的基本原理，并讨论生物打印组织构建体的基本设计原则。接下来，我们描述在许多不同方面进行组织3D生物打印的基本原理和关键考虑因素。此后，我们概述了用于骨科组织工程应用的3D生物打印技术的最新进展，以及所使用的工程方法和材料的详细策略，并讨论了不同3D生物打印组织产品的可能性和局限性。最后，我们总结了3D生物打印技术在骨科组织工程和再生医学中的当前挑战和未来方向。本综述不仅描述了代表性的3D生物打印策略及其组织工程应用, 还为3D生物打印组织的临床转化提供了新的见解，以帮助推动骨科植入物的未来发展。重要性声明：3D生物打印推动了组织工程和再生医学领域的重大创新；旨在开发一种功能性可行的组织构建体，为肌肉骨骼组织再生提供替代的再生疗法。基于3D生物打印的生物制造策略可以为创建等效的组织替代物开辟新的临床可能性，并能够根据患者需求对其进行定制。在本综述中，我们总结了3D生物打印技术和先进生物墨水的重要性和最新进展。我们强调了使用3D生物打印进行骨科组织工程应用的生物制造策略的基本原理。此外，我们对骨科生物打印转化研究的当前挑战和未来方向提供了充分的观点和新的见解。
