College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, China.
Haide College, Ocean University of China, Qingdao, China.
Virus Res. 2024 Jan 2;339:199270. doi: 10.1016/j.virusres.2023.199270. Epub 2023 Nov 18.
Vibrio is a prevalent bacterial genus in aquatic environments and exhibits diverse metabolic capabilities, playing a vital role in marine biogeochemical cycles. This study isolated a novel virus infecting Vibrio cyclitrophicus, vB_VviC_ZQ26, from coastal waters near Qingdao, China. The vB_VviC_ZQ26 comprises a linear double-stranded DNA genome with a length of 42,982 bp and a G + C content of 43.21 %, encoding 72 putative open reading frames (ORFs). Transmission electron microscope characterization indicates a siphoviral-morphology of vB_VviC_ZQ26. Nucleic-acids-wide analysis indicates a tetranucleotide frequency deviation for genomic segments encoding putative gene transfer agent protein (GTA) and coil-containing protein, implying divergent origins occurred in different parts of viral genomes. Phylogenetic and genome-content-based analysis suggest that vB_VviC_ZQ26 represents a novel vibriophage-specific family designated as Coheviridae. From the result of biogeographic analysis, Coheviridae is mainly colonized in the temperate and tropical epipelagic zones. This study describes a novel vibriophage infecting V. cyclitrophicus, shedding light on the evolutionary divergence of different parts of the viral genome and its ecological footprint in marine environments.
弧菌是水生环境中普遍存在的细菌属，具有多样化的代谢能力，在海洋生物地球化学循环中起着至关重要的作用。本研究从中国青岛附近的沿海水域中分离到一株感染环指状(Vibrio cyclitrophicus)的新型病毒，命名为 vB_VviC_ZQ26。vB_VviC_ZQ26 的基因组为线性双链 DNA，全长 42982bp，G+C 含量为 43.21%，编码 72 个潜在的开放阅读框（ORFs）。透射电镜观察表明，vB_VviC_ZQ26 具有长尾噬菌体的形态特征。全基因组核苷酸序列分析表明，编码转导相关蛋白（GTA）和卷曲蛋白的基因组片段存在四核苷酸频率偏差，提示这些基因在病毒基因组的不同区域可能具有不同的起源。基于系统发育和基因组内容的分析，vB_VviC_ZQ26 代表了一种新型的、专门感染弧菌的噬菌体科，命名为 Coheviridae 科。生物地理分析的结果表明，Coheviridae 科主要分布在温带和热带表水层。本研究描述了一株感染 V. cyclitrophicus 的新型弧菌噬菌体，揭示了病毒基因组不同区域的进化分歧及其在海洋环境中的生态足迹。
