College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, MoE Laboratory of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, China.
Haide College, Ocean University of China, Qingdao, China.
Int Microbiol. 2024 Aug;27(4):1297-1306. doi: 10.1007/s10123-023-00476-5. Epub 2024 Jan 8.
Sulfitobacter is a bacterium recognized for its production of AMP-independent sulfite oxidase, which is instrumental in the creation of sulfite biosensors. This capability underscores its ecological and economic relevance. In this study, we present a newly discovered phage, Sulfitobacter phage vB_SupP_AX, which was isolated from Maidao of Qingdao, China. The vB_SupP_AX genome is linear and double-stranded and measures 75,445 bp with a GC content of 49%. It encompasses four transfer RNA (tRNA) sequences and 79 open reading frames (ORFs), one of which is an auxiliary metabolic gene encoding thioredoxin. Consistent with other N4-like phages, vB_SupP_AX possesses three distinct RNA polymerases and is characterized by the presence of four tRNA molecules. Comparative genomic and phylogenetic analyses position vB_SupP_AX and three other viral genomes from the Integrated Microbial Genomes/Virus v4 database within the Rhodovirinae virus subfamily. The identification of vB_SupP_AX enhances our understanding of virus-host interactions within marine ecosystems.
硫酸盐还原菌是一种以产生 AMP 非依赖性亚硫酸盐氧化酶而闻名的细菌，这种酶在亚硫酸盐生物传感器的创建中起着关键作用。这种能力突显了它在生态和经济方面的重要性。在这项研究中，我们介绍了一种新发现的噬菌体，硫酸盐还原菌噬菌体 vB_SupP_AX，它是从中国青岛的麦岛分离出来的。vB_SupP_AX 的基因组是线性的，双链的，长 75445bp，GC 含量为 49%。它包含四个转移 RNA(tRNA)序列和 79 个开放阅读框(ORFs)，其中一个是编码硫氧还蛋白的辅助代谢基因。与其他 N4 样噬菌体一样，vB_SupP_AX 拥有三个不同的 RNA 聚合酶，其特征是有四个 tRNA 分子。比较基因组学和系统发育分析将 vB_SupP_AX 与 Integrated Microbial Genomes/Virus v4 数据库中的其他三个病毒基因组定位在 Rhodovirinae 病毒亚科内。vB_SupP_AX 的鉴定增强了我们对海洋生态系统中病毒-宿主相互作用的理解。
