College of Marine Life Sciences, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Institute of Evolution and Marine Biodiversity, Ocean University of Chinagrid.4422.0, Qingdao, China.
UMT-OUC Joint Centre for Marine Studies, Qingdao, China.
Microbiol Spectr. 2022 Aug 31;10(4):e0058522. doi: 10.1128/spectrum.00585-22. Epub 2022 Jul 6.
Vibrio parahaemolyticus, a widespread marine bacterium, is responsible for a variety of diseases in marine organisms. Consumption of raw or undercooked seafood contaminated with V. parahaemolyticus is also known to cause acute gastroenteritis in humans. While numerous dsDNA vibriophages have been isolated so far, there have been few studies of vibriophages belonging to the ssDNA family. In this study, a novel ssDNA phage, vB_VpaM_PG19 infecting V. parahaemolyticus, with a 5,572 bp ssDNA genome with a G+C content of 41.31% and encoded eight open reading frames, was isolated. Genome-wide phylogenetic analysis of the total phage isolates in the GenBank database revealed that vB_VpaM_PG19 was only related to the recently deposited vibriophage vB_VpP_WS1. The genome-wide average nucleotide homology of the two phages was 89.67%. The phylogenetic tree and network analysis showed that vB_VpaM_PG19 was different from other members of the family and might represent a novel viral genus, together with vibriophage vB_VpP_WS1, named . One-step growth curves showed that vB_VpaM_PG19 has a short incubation period, suggesting its potential as an antimicrobial agent for pathogenic V. parahaemolyticus. Vibriophage vB_VpaM_PG19 was distant from other isolated microviruses in the phylogenetic tree and network analysis and represents a novel microviral genus, named . Our report describes the genomic and phylogenetic features of vB_VpaM_PG19 and provides a potential antimicrobial candidate for pathogenic V. parahaemolyticus.
副溶血弧菌，一种广泛存在的海洋细菌，可导致多种海洋生物疾病。食用受副溶血弧菌污染的生的或未煮熟的海鲜，已知也会导致人类急性肠胃炎。虽然迄今为止已经分离出了许多双链 DNA 噬菌体，但对属于单链 DNA 家族的噬菌体的研究却很少。在这项研究中，分离到一种新型单链 DNA 噬菌体 vB_VpaM_PG19，可感染副溶血弧菌，其基因组为 5572bp 的单链 DNA，G+C 含量为 41.31%，编码 8 个开放阅读框。对 GenBank 数据库中总噬菌体分离株的全基因组系统发育分析表明，vB_VpaM_PG19 仅与最近提交的噬菌体 vB_VpP_WS1 有关。两噬菌体的全基因组平均核苷酸同源性为 89.67%。系统发育树和网络分析表明，vB_VpaM_PG19 与 家族的其他成员不同，可能与噬菌体 vB_VpP_WS1 一起代表一个新的病毒属，命名为 。一步生长曲线表明，vB_VpaM_PG19 的潜伏期很短，这表明它可能是一种针对致病性副溶血弧菌的抗菌剂。噬菌体 vB_VpaM_PG19 在系统发育树和网络分析中与其他分离的微噬菌体相距较远，代表了一个新的微噬菌体属，命名为 。本报告描述了 vB_VpaM_PG19 的基因组和系统发育特征，并为致病性副溶血弧菌提供了一种潜在的抗菌候选物。
