红细胞谷胱甘肽耗竭会损害饮酒女性对溶血的抵抗力。

Erythrocyte glutathione depletion impairs resistance to haemolysis in women consuming alcohol.

机构信息

P.G and Research Department Of Biochemistry, Bharathi Women’s College, Affiliated to the University of Madras, Chennai-600 108, Tamilnadu, India.

出版信息

J Clin Biochem Nutr. 2008 Jan;42(1):14-20. doi: 10.3164/jcbn.2008003.

DOI:10.3164/jcbn.2008003

PMID:18231625

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2212343/

Abstract

Alcohol abuse is known to cause an array of ethanol induced abnormalities in men but very few reports are available on the effect of alcohol in women. None of them discuss the effect of ethanol consumption on erythrocyte membrane. In the present study, erythrocytes in women who consume alcohol showed significant decrease in their ability to resist haemolysis with HPLC studies. Erythrocyte membrane indicates decreased phospholipid (p<0.05) levels, which increased the cholesterol/phospholipid ratio significantly (p<0.01) in women who consume alcohol. This can decrease the fluidity of membrane, which appears to be related to the effect of ethanol on erythrocyte membrane. Also the protection against exogenous and endogenous peroxides in the erythrocytes of alcoholic women is considerably affected due to decreased (p<0.05) activity of catalase, glucose-6-phosphate dehydrogenase, protein-SH group and glutathione (GSH). Enhanced free radical generation induced oxidation of oxyHb to metHb in alcoholics. Increased methemoglobin leads to significant reduction in membrane GSH, which may cause protein thiol oxidation. Thus peroxidative damage to membrane lipids and oxidation of membrane protein thiols potentially harmful to membrane fluidity and flexibility is responsible for decreased resistance to haemolysis as demonstrated in women who consume alcohol.

摘要

酗酒已知会导致男性出现一系列乙醇引起的异常，但关于酒精对女性影响的报道却很少。这些报道都没有讨论乙醇摄入对红细胞膜的影响。在本研究中，通过 HPLC 研究发现，饮酒女性的红细胞抵抗溶血的能力显著下降。红细胞膜表明磷脂水平降低（p<0.05），胆固醇/磷脂比值显著增加（p<0.01），这可能会降低膜的流动性，这似乎与乙醇对红细胞膜的影响有关。由于过氧化氢酶、葡萄糖-6-磷酸脱氢酶、蛋白质-SH 基团和谷胱甘肽（GSH）活性降低（p<0.05），饮酒女性红细胞对外源和内源性过氧化物的保护作用受到严重影响。自由基的产生增强会诱导氧合血红蛋白氧化为高铁血红蛋白，增加的高铁血红蛋白会导致膜 GSH 显著减少，这可能导致蛋白质巯基氧化。因此，膜脂质的过氧化损伤和膜蛋白巯基的氧化，可能会对膜的流动性和柔韧性造成潜在的危害，从而导致饮酒女性红细胞对溶血的抵抗力下降，正如我们在研究中所观察到的那样。