Department of Chemical and Biomolecular Engineering (BK21 Four), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Center for Infectious Disease Vaccine and Diagnosis Innovation, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea.
Anal Chem. 2023 Dec 5;95(48):17629-17636. doi: 10.1021/acs.analchem.3c03313. Epub 2023 Nov 17.
We herein describe a novel centrifugal microfluidic system to achieve multiple standard additions, which could minimize the effects of matrix interference and consequently lead to more accurate and reliable measurements of analyte concentrations in complex samples. The system leverages laser-irradiated ferrowax microvalves to automatically control fluid transfer on the disc without the need for external pumps or pressure systems, simplifying the procedures and eliminating the need for manual intervention. The disc incorporates metering chambers with rationally designed varying sizes, which could lead to the formation of six standard addition samples very rapidly in just 2.5 min. The final solutions are designed to contain a target component at gradually increasing concentrations but have an equal final volume containing the same amount of an analyte solution, thereby equalizing the matrix effect that is supposedly caused by the unknown components in the analyte solution. By utilizing this design principle, we were able to successfully quantify a model target component, salivary thiocyanate ions, that could be used as a biomarker for exposure to tobacco smoke. Our centrifugal microfluidic system holds great promise as a powerful analytical tool to achieve fully automated diagnostic microsystems involving a standard addition process.
我们在此描述了一种新颖的离心微流控系统，可实现多次标准加入，从而最大程度地减少基质干扰的影响，进而更准确、可靠地测量复杂样品中的分析物浓度。该系统利用激光照射的铁蜡微阀在不依赖外部泵或压力系统的情况下自动控制盘上的流体转移，简化了操作步骤，且无需人工干预。该盘上的计量室具有合理设计的不同大小，可以在短短 2.5 分钟内快速形成 6 个标准加入样品。最终溶液设计为含有目标成分的浓度逐渐增加，但最终体积相同，均含有相同量的分析物溶液，从而使分析物溶液中未知成分引起的基质效应均等化。通过利用这一设计原理，我们成功地定量了一种模型目标成分——唾液硫氰酸盐离子，它可作为暴露于烟草烟雾的生物标志物。我们的离心微流控系统有望成为一种强大的分析工具，用于实现涉及标准加入过程的全自动诊断微系统。
