Department of Biology and Control of Disease Vectors, School of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Acta Parasitol. 2024 Mar;69(1):216-226. doi: 10.1007/s11686-023-00735-5. Epub 2023 Nov 18.
The use of synthetic pesticides to control the spread of mosquito-borne diseases has caused environmental pollution and insecticide resistance in mosquitoes. Developments of new green insecticides have thus received more attention to overcome these problems.
Nanoliposomes containing carvone and essential oils were first prepared. The nanoliposome physicochemical characteristics (particle size, morphology, and successful loading) were then evaluated by Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), and the Attenuated Total Reflection-Fourier Transform InfraRed (ATR-FTIR) analyses. Larvicidal effects of carvone, Mentha spicata, and Tanacetum balsamita essential oils were investigated against the main malaria vector, Anopheles stephensi, in non-formulated and nanoformulated states.
The larvicidal effects of nanoformulated states were significantly more potent (7.2 folds, 3.5 folds, and 8 folds) than non-formulated states. Nanoliposomes containing M. spicata and T. balsamita essential oils with particle sizes of 175 ± 8 and 184 ± 5 nm showed the best efficacies (LC50 values = 9.74 and 9.36 μg/mL).
The prepared samples could be used as new green potent larvicides against An stephensi mosquito in further field trials. It is also recommended to investigate their efficacies against other mosquitoes.
利用合成杀虫剂来控制蚊媒疾病的传播，已导致环境污染和蚊子对杀虫剂产生抗药性。因此，开发新的绿色杀虫剂受到了更多关注，以克服这些问题。
首先制备含有香芹酮和精油的纳米脂质体。然后通过动态光散射（DLS）、透射电子显微镜（TEM）和衰减全反射傅里叶变换红外（ATR-FTIR）分析来评估纳米脂质体的理化特性（粒径、形态和成功负载）。研究了香芹酮、薄荷和母菊精油在非配方和纳米配方状态下对主要疟疾传播媒介按蚊的杀幼虫效果。
纳米配方状态的杀幼虫效果明显更强（7.2 倍、3.5 倍和 8 倍）。粒径为 175±8nm 和 184±5nm 的含薄荷和母菊精油的纳米脂质体显示出最佳功效（LC50 值分别为 9.74μg/mL 和 9.36μg/mL）。
所制备的样品可作为新型绿色高效杀幼虫剂，进一步在野外试验中用于防治按蚊。建议进一步研究它们对其他蚊子的功效。
