College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, Anhui, China.
Int J Biol Macromol. 2024 Jan;255:127942. doi: 10.1016/j.ijbiomac.2023.127942. Epub 2023 Nov 17.
A systematic analysis of genes related to reproduction is crucial for obtaining a comprehensive understanding of the molecular mechanisms that underlie male reproductive traits in mammals. Here, we utilized 435 goat transcriptome datasets to unveil the testicular tissue-specific genes (TSGs), allele-specific expression (ASE) genes and their uncharacterized transcriptional features related to male goat reproduction. Results showed a total of 1790 TSGs were identified in goat testis, which was the most among all tissues. GO enrichment analyses suggested that testicular TSGs were mainly involved in spermatogenesis, multicellular organism development, spermatid development, and flagellated sperm motility. Subsequently, a total of 95 highly conserved TSGs (HCTSGs), 508 middle conserved TSGs (MCTSGs) and 42 no conserved TSGs (NCTSGs) were identified in goat testis. GO enrichment analyses suggested that the HCTSGs and MCTSGs has a more important association with male reproduction than NCTSGs. Additionally, we identified 644 ASE genes, including 88 tissue-specific ASE (TS-ASE) genes (e.g., FSIP2, TDRD9). GO enrichment analyses indicated that both ASE genes and TS-ASE genes were associated with goat male reproduction. Overall, this study revealed an extensive gene set involved in the regulation of male goat reproduction and their dynamic transcription patterns. Data reported here provide valuable insights for a further improvement of the economic benefits of goats as well as future treatments for male infertility.
对与生殖相关的基因进行系统分析对于全面了解哺乳动物雄性生殖特征的分子机制至关重要。在这里，我们利用 435 个山羊转录组数据集揭示了睾丸组织特异性基因（TSGs）、等位基因特异性表达（ASE）基因及其与雄性山羊繁殖相关的未表征转录特征。结果表明，在山羊睾丸中共鉴定出 1790 个 TSGs，在所有组织中数量最多。GO 富集分析表明，睾丸 TSGs 主要参与精子发生、多细胞生物发育、精子细胞发育和鞭毛精子运动。随后，在山羊睾丸中共鉴定出 95 个高度保守的 TSGs（HCTSGs）、508 个中度保守的 TSGs（MCTSGs）和 42 个无保守的 TSGs（NCTSGs）。GO 富集分析表明，HCTSGs 和 MCTSGs 与雄性生殖的相关性比 NCTSGs 更为重要。此外，我们鉴定出 644 个 ASE 基因，包括 88 个组织特异性 ASE（TS-ASE）基因（如 FSIP2、TDRD9）。GO 富集分析表明，ASE 基因和 TS-ASE 基因都与山羊雄性生殖有关。总的来说，这项研究揭示了广泛的基因集参与调节雄性山羊生殖及其动态转录模式。本研究提供了有价值的见解，有助于进一步提高山羊的经济效益和未来男性不育的治疗。
