School of Nutritional Sciences and Wellness, University of Arizona, Tucson, AZ, United States of America.
Biostatistics and Bioinformatics Shared Resource, Arizona Cancer Center, University of Arizona, Tucson, AZ, United States of America.
PLoS One. 2023 Nov 20;18(11):e0294355. doi: 10.1371/journal.pone.0294355. eCollection 2023.
Salivary gland hypofunction is an adverse side effect associated with radiotherapy for head and neck cancer patients. This study delineated metabolic changes at acute, intermediate, and chronic radiation damage response stages in mouse salivary glands following a single 5 Gy dose. Ultra-high performance liquid chromatography-mass spectrometry was performed on parotid salivary gland tissue collected at 3, 14, and 30 days following radiation (IR). Pathway enrichment analysis, network analysis based on metabolite structural similarity, and network analysis based on metabolite abundance correlations were used to incorporate both metabolite levels and structural annotation. The greatest number of enriched pathways are observed at 3 days and the lowest at 30 days following radiation. Amino acid metabolism pathways, glutathione metabolism, and central carbon metabolism in cancer are enriched at all radiation time points across different analytical methods. This study suggests that glutathione and central carbon metabolism in cancer may be important pathways in the unresolved effect of radiation treatment.
唾液腺功能低下是头颈部癌症患者放射治疗的一种不良反应。本研究描绘了单次 5Gy 剂量照射后，小鼠唾液腺在急性、中期和慢性放射损伤反应阶段的代谢变化。对放射后 3、14 和 30 天收集的腮腺唾液腺组织进行超高效液相色谱-质谱分析（IR）。采用基于代谢物结构相似性的通路富集分析、基于代谢物丰度相关性的网络分析以及基于代谢物水平和结构注释的网络分析。在放射后 3 天观察到的富集途径数量最多，在 30 天观察到的途径数量最少。在不同的分析方法中，所有放射时间点均富集了氨基酸代谢途径、谷胱甘肽代谢和癌症中的中心碳代谢。本研究表明，谷胱甘肽和癌症中的中心碳代谢可能是放射治疗未解决效应的重要途径。
