Department of Pharmacy, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, P. R. China.
Department of Neurology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, P. R. China.
Brain Behav. 2023 Dec;13(12):e3318. doi: 10.1002/brb3.3318. Epub 2023 Nov 20.
It is estimated that 11.5% of patients with stroke (STR) were at risk of suffering poststroke epilepsy (PSE) within 5 years. Gut microbiota is shown to affect health in humans by producing metabolites. The association between dysregulation of gut microbiota and STR/PSE remains unclear. The aim of this study was to identify potential gut microbiota and functional component in STR and PSE, which may provide a theoretical foundation for diagnosis and treatment of STR and PSE.
The fresh stool samples were collected from 19 healthy controls, 27 STR patients, and 20 PSE patients for 16S rRNA gene sequencing. Analysis of amplicon sequence variant and community diversity was performed, followed by the identification of dominant species, species differences analysis, diagnostic, and functional analysis of species in STR and PSE.
Community diversity was decreased in STR and PSE. Some disordered profiles of gut microbiota in STR and PSE were identified, such as the increase of Enterococcus and the decrease of butyricicoccus in STR, the increase of Escherichia Shigella and Clostridium innocuum-group and the decrease of Faecalibacterium in PSE, and the decrease of Anaerostipes in both STR and PSE. Moreover, potential diagnostic biomarkers for STR (butyricicoccus), PSE (Faecalibacterium), STR, and PSE (NK4A214_group and Veillonella) were identified. Several significantly dysfunctional components were identified, including l-tryptophan biosynthesis in STR, fatty acid biosynthesis in PSE, and Stress_Tolerant and anaerobic in both STR and PSE.
The disturbed gut microbiota and related dysfunctional components are closely associated with the progression of STR and PSE.
据估计，5 年内有 11.5%的中风（STR）患者有发生中风后癫痫（PSE）的风险。肠道微生物群通过产生代谢物来影响人类健康。肠道微生物群失调与 STR/PSE 之间的关系尚不清楚。本研究旨在确定 STR 和 PSE 中潜在的肠道微生物群和功能成分，为 STR 和 PSE 的诊断和治疗提供理论基础。
从 19 名健康对照者、27 名 STR 患者和 20 名 PSE 患者中采集新鲜粪便样本进行 16S rRNA 基因测序。对扩增子序列变异和群落多样性进行分析，然后鉴定优势物种、物种差异分析、STR 和 PSE 中物种的诊断和功能分析。
STR 和 PSE 中的群落多样性降低。在 STR 和 PSE 中发现了一些肠道微生物群失调的特征谱，例如 STR 中肠球菌的增加和丁酸球菌的减少，PSE 中大肠杆菌和志贺氏菌以及 innocuum 群和粪杆菌的增加，以及两者中厌氧性梭菌的减少。此外，还鉴定出 STR（丁酸球菌）、PSE（粪杆菌）、STR 和 PSE（NK4A214 群和韦荣球菌）的潜在诊断生物标志物。鉴定出几个功能失调的成分，包括 STR 中的 l-色氨酸生物合成、PSE 中的脂肪酸生物合成以及 STR 和 PSE 中的应激耐受和厌氧。
紊乱的肠道微生物群及其相关功能失调成分与 STR 和 PSE 的进展密切相关。
